SPO, Univ Montpellier, INRAE, Institut Agro, Montpellier, France.
Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France.
Genome Biol Evol. 2022 Feb 4;14(2). doi: 10.1093/gbe/evac007.
Recent studies have suggested that species of the Kazachstania genus may be interesting models of yeast domestication. Among these, Kazachstania barnettii has been isolated from various microbially transformed foodstuffs such as sourdough bread and kefir. In the present work, we sequence, assemble, and annotate the complete genomes of two K. barnettii strains: CLIB 433, being one of the two reference strains for K. barnettii that was isolated as a spoilage organism in soft drink, and CLIB 1767, recently isolated from artisan bread-making sourdough. Both assemblies are of high quality with N50 statistics greater than 1.3 Mb and BUSCO score greater than 99%. An extensive comparison of the two obtained genomes revealed very few differences between the two K. barnettii strains, considering both genome structure and gene content. The proposed genome assemblies will constitute valuable references for future comparative genomic, population genomic, or transcriptomic studies of the K. barnettii species.
最近的研究表明,Kazachstania 属的某些物种可能是酵母驯化的有趣模型。在这些物种中,Kazachstania barnettii 已从各种微生物转化的食品中分离出来,如酸面团面包和克菲尔。在本工作中,我们对两株 K. barnettii 菌株的完整基因组进行了测序、组装和注释:CLIB 433 是作为软饮料中腐败菌分离出来的 K. barnettii 的两个参考菌株之一,CLIB 1767 最近则从手工面包制作的酸面团中分离出来。两个基因组组装的质量都很高,N50 统计值大于 1.3Mb,BUSCO 评分为大于 99%。对这两个获得的基因组进行的广泛比较表明,考虑到基因组结构和基因含量,这两个 K. barnettii 菌株之间几乎没有差异。所提出的基因组组装将为未来 K. barnettii 物种的比较基因组学、群体基因组学或转录组学研究提供有价值的参考。