Prieto-Santos M I, Martín-Checa J, Balaña-Fouce R, Garrido-Pertierra A
Biochim Biophys Acta. 1986 Feb 19;880(2-3):242-4. doi: 10.1016/0304-4165(86)90085-1.
Escherichia coli mutants able to grow in putrescine have been isolated from gamma-aminobutyrate mutants. These mutants show putrescine-alpha-ketoglutarate transaminase and gamma-aminobutyraldehyde dehydrogenase activities. Both enzymes have been characterized, the first of them showing an apparent Km for putrescine of 22.5 microM and the second an apparent Km of 37 microM for NAD and 18 microM for delta-1-pyrroline; the optimum pH values were 7.2 and 5.4, respectively, for the two enzymes.
能够在腐胺中生长的大肠杆菌突变体已从γ-氨基丁酸突变体中分离出来。这些突变体表现出腐胺-α-酮戊二酸转氨酶和γ-氨基丁醛脱氢酶活性。两种酶均已得到表征，第一种酶对腐胺的表观Km为22.5微摩尔，第二种酶对NAD的表观Km为37微摩尔，对δ-1-吡咯啉的表观Km为18微摩尔；两种酶的最适pH值分别为7.2和5.4。
