Division of Biochemistry, Department of Oral Biology, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu-cho, Hokkaido, Japan.
Division of Orthodontics and Dentofacial Orthopedics, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu-cho, Hokkaido, Japan.
Clin Exp Dent Res. 2022 Jun;8(3):658-672. doi: 10.1002/cre2.533. Epub 2022 Feb 1.
The periodontal ligament (PDL) is an important component of periodontium to support dental structure in the alveolar socket. Regeneration of PDL tissue is an effective treatment option for periodontal disease and the profiling of genes involved in this process will be informative. Therefore, our study aims to accurately delineate the profiling of gene expression for PDL tissue regeneration.
We isolated PDL tissues and PDL fibroblasts (PDLFs) from premolar teeth, which were extracted from healthy periodontal status patients undergoing orthodontic treatment. Messenger RNA (mRNA) expression in PDL tissue and PDLFs were analyzed using Cap analysis gene expression, which is a second-generation sequencing technique to create profiling. We also determined the protein expression using Western blot.
Collagens (type I, III, and VI), noncollagenous proteins (periostin and osteonectin), and proteoglycans (asporin, lumican, decorin, and osteomodulin) were highly expressed in PDL tissue. Integrin, β1 was also expressed in PDL tissue. On comparison of gene expression between PDL tissue and PDLFs, four PDL marker genes, osteopontin, asporin, periostin, and osteonectin, were decreased in PDLFs. The genes for gene regulation were also highly expressed.
Our study demonstrated the overall profiling of mRNA expression in PDL tissue and analyzed the important genes which may be useful for providing specific information for the reconstruction of PDL. We also identified the difference in gene expression between PDL tissue and PDLFs which might provide insights towards PDL regeneration.
牙周韧带（PDL）是牙周组织的重要组成部分，可支撑牙槽窝中的牙齿结构。PDL 组织再生是牙周病的有效治疗选择，对涉及该过程的基因进行分析将提供有价值的信息。因此，我们的研究旨在准确描绘 PDL 组织再生的基因表达谱。
我们从接受正畸治疗的健康牙周状态患者的前磨牙中分离出 PDL 组织和 PDL 成纤维细胞（PDLFs）。使用 Cap 分析基因表达（一种第二代测序技术来创建基因表达谱）分析 PDL 组织和 PDLFs 的信使 RNA（mRNA）表达。我们还使用 Western blot 确定蛋白质表达。
胶原（I 型、III 型和 VI 型）、非胶原蛋白（骨膜蛋白和骨连蛋白）和蛋白聚糖（聚集蛋白聚糖、核心蛋白聚糖、饰胶蛋白聚糖和骨调节素）在 PDL 组织中高表达。整合素β1也在 PDL 组织中表达。与 PDL 组织和 PDLFs 之间的基因表达比较，PDLFs 中 4 个 PDL 标记基因（骨桥蛋白、聚集蛋白聚糖、骨膜蛋白和骨连蛋白）表达下调。基因调控相关基因也高表达。
本研究展示了 PDL 组织中 mRNA 表达的总体谱，并分析了可能为 PDL 重建提供特定信息的重要基因。我们还确定了 PDL 组织和 PDLFs 之间基因表达的差异，这可能为 PDL 再生提供新的见解。
