University of Gaziantep, Department of Physiology, Medical Faculty, Health Sciences Institution, 27310, Gaziantep, Turkey.
University of Fırat, De partment of Pathology, Faculty of Veterinary, 23119, Elazığ, Turkey.
An Acad Bras Cienc. 2022 Jan 31;94(1):e20201562. doi: 10.1590/0001-3765202120201562. eCollection 2022.
This study aimed to investigate the effects of Extracellular Vesicles (EV) secreted from mouse embryonic fibroblasts EV on wound healing of full-thickness skin defect in a diabetic mouse model. The study included both in vitro and in vivo experimental studies 82 mice. In the in vitro stage of the experimental study, hysterectomy was performed on two mice between the 13-14th days of pregnancy and then EV was isolated by cell culturing. VEGF, IL-6, and TNF-α biomarkers were examined in tissue homogenate. Moreover, tissue taken from wound area was also subjected to histopathologic scoring. EV augmented the effect of VEGF. Therefore promoted angiogenesis increases the transport of cells, essential oxygen and nutrients in the wound area. Extracellular vesicles isolated from mouse embryonic fibroblasts have been found to accelerate wound healing in diabetes. The findings obtained from this experimental study indicate that EV isolated from mouse embryonic fibroblasts accelerate the wound healing process in experimentally induced-diabetes in mice.
本研究旨在探讨从鼠胚胎成纤维细胞 EV 分泌的细胞外囊泡 (EV) 对糖尿病小鼠全层皮肤缺损创面愈合的影响。研究包括两项实验，共 82 只小鼠。在实验研究的体外阶段，对怀孕第 13-14 天的两只小鼠进行了子宫切除术，然后通过细胞培养分离 EV。在组织匀浆中检测 VEGF、IL-6 和 TNF-α 生物标志物。此外，还对取自创面的组织进行了组织病理学评分。EV 增强了 VEGF 的作用。因此促进了血管生成，增加了细胞、必需氧气和营养物质在创面的运输。从鼠胚胎成纤维细胞中分离出的细胞外囊泡已被发现可加速糖尿病患者的伤口愈合。这项实验研究的结果表明，从鼠胚胎成纤维细胞中分离出的 EV 可加速实验性诱导的糖尿病小鼠的伤口愈合过程。
