Department of Endodontics, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Molecular Medicine and Genetics, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Mol Biol Rep. 2022 May;49(5):3685-3692. doi: 10.1007/s11033-022-07208-4. Epub 2022 Feb 2.
This study assessed the effect of Biodentine coated with Emdogain (Biodentine/Emdogain) on proliferation and differentiation of human stem cells from the apical papilla (SCAPs). METHODS AND RESULTS: In this in vitro, experimental study, SCAPs were isolated from two immature impacted third molars and cultured. After ensuring the stemness of the cells by assessing the cell surface markers, they were exposed to Biodentine, Emdogain, and Biodentine/Emdogain for 24 and 72 h. The control cells did not receive any intervention. Cell viability was evaluated by the methyl thiazolyl tetrazolium assay. Expression of odontogenic differentiation genes was analyzed by the quantitative reverse transcription polymerase chain reaction. Alkaline phosphatase (ALP) activity was quantified by the respective kit. Data were analyzed by one-way ANOVA, t-test, and Mann-Whitney test (α = 0.05). Cell viability did not change after 24 h of exposure to biomaterials. At 72 h, the viability of the cells exposed to Biodentine and Biodentine/Emdogain decreased compared with the control group. The expression of dentin sialophosphoprotein, dentin matrix protein 1, and bone sialoprotein genes, and ALP activity significantly increased in all three experimental groups, compared with the control group at both 24 and 72 h; this increase was significantly greater in Biodentine/Emdogain group. The number of mineralized nodules significantly increased in all groups after 72 h with a greater rate in Biodentine/Emdogain group.
All biomaterials increased the differentiation of SCAPs, expression of odontogenic genes, and ALP activity, but Biodentine/Emdogain was significantly more effective for this purpose.
本研究评估了 Biodentine 涂层 Emdogain（Biodentine/Emdogain）对人根尖乳头干细胞（SCAPs）增殖和分化的影响。
在这项体外实验研究中，从两颗未成熟的埋伏第三磨牙中分离出 SCAPs 并进行培养。通过评估细胞表面标志物来确保细胞的干性后，将其暴露于 Biodentine、Emdogain 和 Biodentine/Emdogain 中 24 和 72 小时。对照组细胞未接受任何干预。通过甲基噻唑基四唑测定法评估细胞活力。通过定量逆转录聚合酶链反应分析牙源性分化基因的表达。通过各自的试剂盒定量碱性磷酸酶（ALP）活性。数据通过单因素方差分析、t 检验和曼-惠特尼检验（α=0.05）进行分析。暴露于生物材料 24 小时后，细胞活力没有变化。72 小时后，暴露于 Biodentine 和 Biodentine/Emdogain 的细胞活力与对照组相比降低。与对照组相比，在 24 和 72 小时时，所有三个实验组的牙本质涎磷蛋白、牙本质基质蛋白 1 和骨涎蛋白基因的表达以及 ALP 活性均显著增加；Biodentine/Emdogain 组的增加更为显著。72 小时后，所有组的矿化结节数量均显著增加，Biodentine/Emdogain 组的增加速度更快。
所有生物材料均能促进 SCAPs 的分化、牙源性基因的表达和 ALP 活性，但 Biodentine/Emdogain 的效果更为显著。
