生物陶瓷材料对根尖乳头人干细胞增殖和成牙本质细胞分化的影响。
Effect of Bioceramic Materials on Proliferation and Odontoblast Differentiation of Human Stem Cells from the Apical Papilla.
机构信息
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
出版信息
J Endod. 2018 Aug;44(8):1270-1275. doi: 10.1016/j.joen.2018.03.014. Epub 2018 Jun 20.
INTRODUCTION
In regenerative endodontic treatment (RET), practitioners favor the placement of bioceramics as sealing materials over blood clots. It is important to understand the interaction between sealing material and cells in the root canal. The purpose of this study was to compare the effectiveness of various bioceramic materials (ProRoot MTA [Dentsply, Tulsa, OK], Biodentine [Septodont, Saint-Maur-des-Fossés, France], and RetroMTA [BioMTA, Seoul, Korea]) as sealing materials in RET for the proliferation and differentiation of stem cells from the apical papilla (SCAPs).
METHODS
SCAPs were seeded at 20,000 cells/well and cultured with soluble agents of testing materials through a transwell culture plate. The proliferation of SCAPs was investigated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on days 1, 3, 7, and 14 of testing. Alizarin red staining and quantitative real-time polymerase chain reaction were used for SCAP differentiation at different time points (1, 7, 14, and 21 days). The odontoblast genes expressed are dentin matrix acidic phosphoprotein 1, dentin sialophosphoprotein, osteocalcin, and matrix extracellular phosphoglycoprotein, which were used in this study. The SCAPs were cultured in odonto/osteogenic induction medium and also contacted soluble agents from the testing materials.
RESULTS
All 3 tested biomaterials induced SCAP proliferation. The Biodentine, ProRootMTA, and RetroMTA groups showed significant SCAP proliferation on days 7 and 14 compared with the control. In regard to odontoblastic differentiation, only Biodentine showed positive alizarin red staining. The highest expressions of dentin matrix acidic phosphoprotein 1, dentin sialophosphoprotein, and matrix extracellular phosphoglycoprotein were found on day 21 in the Biodentine group. The expression of osteocalcin was found to be significant on day 7.
CONCLUSIONS
Biodentine, ProRootMTA, and RetroMTA can induce SCAP proliferation. Biodentine induced significant SCAP differentiation among the 3 materials.
简介
在再生性牙内治疗（RET）中，从业者倾向于将生物陶瓷作为密封材料，而不是血凝块。了解密封材料与根管内细胞的相互作用非常重要。本研究的目的是比较各种生物陶瓷材料（ProRoot MTA[登士柏，塔尔萨，OK]、Biodentine[赛普敦，圣莫里斯-德-福斯，法国]和 RetroMTA[BioMTA，首尔，韩国]）作为 RET 中用于干细胞增殖和分化的密封材料的效果，这些干细胞来自根尖乳头（SCAPs）。
方法
将 SCAP 以 20000 个细胞/孔的密度接种到 Transwell 培养板中，并通过培养板与测试材料的可溶性试剂进行共培养。在第 1、3、7 和 14 天，通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐法（3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay）来检测 SCAP 的增殖。在不同时间点（1、7、14 和 21 天）使用茜素红染色和定量实时聚合酶链反应（quantitative real-time polymerase chain reaction）检测 SCAP 分化。本研究中使用的牙本质基质酸性磷酸蛋白 1、牙本质涎磷蛋白、骨钙素和基质细胞外磷酸糖蛋白等成牙/成骨基因。将 SCAP 培养在牙/骨诱导培养基中，并与测试材料的可溶性试剂接触。
结果
所有 3 种测试生物材料均诱导 SCAP 增殖。与对照组相比，Biodentine、ProRoot MTA 和 RetroMTA 组在第 7 天和第 14 天显示出明显的 SCAP 增殖。关于成牙本质分化，只有 Biodentine 显示出茜素红染色阳性。在 Biodentine 组中，在第 21 天发现牙本质基质酸性磷酸蛋白 1、牙本质涎磷蛋白和基质细胞外磷酸糖蛋白的表达最高。骨钙素的表达在第 7 天显著。
结论
Biodentine、ProRoot MTA 和 RetroMTA 均可诱导 SCAP 增殖。在这 3 种材料中，Biodentine 诱导了明显的 SCAP 分化。
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