Khoshbin Elham, Karkehabadi Hamed, Salehi Razieh, Farmany Abbas, Najafi Rezvan, Abbasi Roshanak
Department of Endodontics, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Endodontics, Dental Research Center, Avicenna Institute of Clinical Sciences, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran.
Biotechnol Lett. 2025 Feb 5;47(1):24. doi: 10.1007/s10529-024-03557-1.
The study was designed to explore the enhanced impact of nano-hydroxyapatite and emdogain on the survival and osteogenic/odontogenic differentiation of human stem cells isolated from the apical papilla (hSCAPs).
In this in vitro trial, hSCAPS obtained from intact impacted immature third molars were confirmed to have characteristic cell surface markers, then exposed to nanohydroxyapatite, emdogain, and nanohydroxyapatite coated with emdogain for durations of 1-3 days. The survival of apical papilla stem cells was measured using a methyl thiazolyl tetrazolium assay. The quantitative reverse transcription polymerase chain reaction, alkaline phosphatase activity (ALP) and Alizarin red staining were used to evaluate osteogenic-odontogenic differentiation. Analysis of the data was done using one-way ANOVA, t-test, and Mann-Whitney test (α = 0.05).
At 1-3 days, emdogain exhibited no significant impact on the survival of human stem cells from the apical papilla. In contrast, nanohydroxyapatite (α > 0.05) and nanohydroxyapatite coated with emdogain demonstrated a notable reduction in cell survival compared to the control group (α < 0.05). The expression of dentin sialophosphoprotein, dentin matrix protein 1, and bone sialoprotein genes demonstrated a notable increase in the group treated with nanohydroxyapatite coated with emdogain compared to the other groups (α < 0.05), and furthermore, this group exhibited more pronounced mineralized nodules than the other experimental groups.
In contrast to nanohydroxyapatite, Emdogain did not demonstrate a detrimental effect on the survival of hSCAPs. Nanohydroxyapatite, emdogain, and nanohydroxyapatite coated with emdogain increased osteogenic/odontogenic differentiation of hSCAPs.
本研究旨在探讨纳米羟基磷灰石和釉基质蛋白对从根尖乳头分离的人干细胞（hSCAPs）的存活及成骨/成牙分化的增强作用。
在本体外试验中，从完整的未萌出第三磨牙中获取的hSCAPs经确认具有特征性细胞表面标志物，然后分别暴露于纳米羟基磷灰石、釉基质蛋白以及涂有釉基质蛋白的纳米羟基磷灰石中1 - 3天。使用甲基噻唑基四氮唑法检测根尖乳头干细胞的存活率。采用定量逆转录聚合酶链反应、碱性磷酸酶活性（ALP）及茜素红染色评估成骨 - 成牙分化。使用单因素方差分析、t检验和曼 - 惠特尼检验（α = 0.05）进行数据分析。
在1 - 3天内，釉基质蛋白对根尖乳头人干细胞的存活无显著影响。相比之下，纳米羟基磷灰石（α > 0.05）以及涂有釉基质蛋白的纳米羟基磷灰石与对照组相比，细胞存活率显著降低（α < 0.05）。与其他组相比，涂有釉基质蛋白的纳米羟基磷灰石处理组中牙本质涎磷蛋白、牙本质基质蛋白1和骨涎蛋白基因的表达显著增加（α < 0.05），此外，该组比其他实验组表现出更明显的矿化结节。
与纳米羟基磷灰石不同，釉基质蛋白对hSCAPs的存活未显示出有害作用。纳米羟基磷灰石、釉基质蛋白以及涂有釉基质蛋白的纳米羟基磷灰石均可增加hSCAPs的成骨/成牙分化。
