Department of Biology, College of Sciences, Taibah University, P.O Box 42353, Madinah, Saudi Arabia.
Department of Zoology, College of Sciences, King Saud University, P.O Box 11451, Riyadh, Saudi Arabia.
Acta Parasitol. 2022 Jun;67(2):794-801. doi: 10.1007/s11686-022-00520-w. Epub 2022 Feb 2.
In Saudi Arabia, records on molecular identification of tick-borne infections in camels are relatively scarce; few molecular epidemiological studies have been conducted.
This study aimed to find Anaplasma species and Piroplasma spp. in camels from Riyadh and the Eastern Region, Saudi Arabia.
A total of 1369 blood samples were collected from camels from Riyadh and the Eastern Region and analyzed for the DNA of Anaplasma and Piroplasma species by polymerase chain reaction (PCR).
Piroplasma spp. infection was not observed in any of the blood samples. 616 camels (44.99%) were found to be positive for Anaplasma infection by PCR targeting the 16S rRNA and COX1 genes. Six Anaplasma sequences for the 16S rRNA gene (OK481101-OK481106) were deposited in GenBank and six for the COX1 gene (OK490994-OK490999). They showed 98.3% and 62.7% similarities with Anaplasma marginale (A. marginale) detected in Kenya and Brazil, respectively. Phylogenetic studies revealed that the 12 sequences reported in this study were closely related; they were found in the same cluster as A. marginale isolates previously recorded in South Africa, Brazil, USA, China, and Israel.
Finally, 12 Anaplasma sequences closely related to A. marginale were detected in camels in Riyadh and the Eastern Region, Saudi Arabia. Camels in these areas were confirmed to be free of Piroplasma.
在沙特阿拉伯，有关蜱传感染的分子鉴定记录相对较少；很少有进行分子流行病学研究。
本研究旨在发现沙特阿拉伯利雅得和东部地区骆驼中的无形体属和梨形虫属。
共采集来自利雅得和东部地区的 1369 份骆驼血液样本，并通过聚合酶链反应（PCR）分析无形体属和梨形虫属的 DNA。
PCR 检测未发现任何血液样本存在梨形虫属感染。针对 16S rRNA 和 COX1 基因的 PCR 检测发现，616 只（44.99%）骆驼呈无形体属感染阳性。6 个 16S rRNA 基因序列（OK481101-OK481106）和 6 个 COX1 基因序列（OK490994-OK490999）已在 GenBank 中注册。它们与在肯尼亚和巴西检测到的边缘无形体（A. marginale）分别具有 98.3%和 62.7%的相似性。系统发育研究表明，本研究报告的 12 个序列密切相关；它们与之前在南非、巴西、美国、中国和以色列记录的边缘无形体分离株在同一聚类中。
最后，在沙特阿拉伯利雅得和东部地区的骆驼中检测到 12 个与边缘无形体密切相关的无形体属序列。这些地区的骆驼被证实无梨形虫属感染。
