Fish Health Management Division, ICAR- Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India.
Fish Health Management Division, ICAR- Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India.
Microb Pathog. 2022 Mar;164:105420. doi: 10.1016/j.micpath.2022.105420. Epub 2022 Jan 30.
Gut microbiome homeostasis is critical in preventing diseases. However, the effect of disease on gut microbiota assembly remains unclear. At present, there are no reports on the composition and functional analysis of intestinal microbiota of Indian major carp, rohu (L. rohita) infected with ectoparasite, Argulus. In this study, we analysed and compared the intestinal microbiota of healthy and Argulus-infected rohu by 16S rRNA amplicon sequencing. Argulus infection could significantly influence the diversity and richness of the gut microbiota. However, abundance of Actinobacteria and Patescibacteria were enriched significantly in Argulus-infected fish. Venn diagram revealed that there were many more unique genera in the infected group as compared to control fish. The genera, Stenotrophomonas and Pirellula were significantly increased in infected fish while the abundance of Reyranella was decreased. LEfSe analysis showed a significant enrichment in abundances of 11 taxa in healthy group and 17 taxa in infected group. Furthermore, genera Rubellimicrobium, Dielma, Hyphomicrobium, Reyranella, Streptomyces and Cloacibacterium performed the best in differentiating between both the groups. Predicted microbiota function by PICRUSt revealed that the gut microbiota of infected fish was mainly associated with enriched synthesis of chitinases, chitin binding proteins, osmoprotectant proteins and sulfatases enzymes. There was a positive association between the structural and functional composition of the gut microbiota. The results indicated that the Argulus infection could affect the intestinal microbiota composition and function of rohu.
肠道微生物组的动态平衡对于预防疾病至关重要。然而，疾病对肠道微生物组组装的影响尚不清楚。目前，尚无关于受外寄生虫鲱鱼虱感染的印度鲤鱼罗非鱼（L. rohita）肠道微生物组成和功能分析的报道。在本研究中，我们通过 16S rRNA 扩增子测序分析和比较了健康和受鲱鱼虱感染罗非鱼的肠道微生物组。鲱鱼虱感染可显著影响肠道微生物组的多样性和丰富度。然而，放线菌门和 Patescibacteria 的丰度在受感染的鱼中显著富集。Venn 图显示，与对照组相比，感染组有更多的特有属。感染组中 Stenotrophomonas 和 Pirellula 的丰度显著增加，而 Reyranella 的丰度降低。LEfSe 分析显示，健康组中有 11 个分类群的丰度显著增加，感染组中有 17 个分类群的丰度显著增加。此外，Rubellimicrobium、Dielma、Hyphomicrobium、Reyranella、Streptomyces 和 Cloacibacterium 等属在区分两组方面表现最佳。PICRUSt 预测的微生物功能显示，受感染鱼的肠道微生物群主要与壳聚糖酶、壳聚糖结合蛋白、渗透保护蛋白和硫酸酯酶的合成有关。肠道微生物群的结构和功能组成之间存在正相关。结果表明，鲱鱼虱感染会影响罗非鱼的肠道微生物组组成和功能。
