山荷叶叶片中的化学成分及其细胞毒性。

Chemical Components in Hedera rhombea Leaves and Their Cytotoxicity.

机构信息

School of Pharmacy, Tokyo University of Pharmacy and Life Sciences.

出版信息

Chem Pharm Bull (Tokyo). 2022;70(2):175-181. doi: 10.1248/cpb.c21-00907.

Abstract

Two novel triterpene glycosides (1 and 2), 17 known triterpene glycosides (3-19), two known flavonoid glycosides (20 and 21), and two known norsesquiterpene glucosides (22 and 23) were isolated from Hedera rhombea (Araliaceae) leaves. The structures of 1 and 2 were determined by spectroscopic analysis, including two-dimensional NMR spectroscopy, and chromatographic analysis of the hydrolyzed products. The cytotoxicity of the isolated triterpene glycosides (1-19) against HL-60 human promyelocytic leukemia cells was evaluated. Compounds 9, 10, and 11 were cytotoxic to HL-60 cells with IC values of 7.2, 21.9, and 32.8 µM, respectively. Other compounds isolated from the leaves were not cytotoxic at sample concentrations of 50 μM.

摘要

从常春藤（五加科）叶子中分离得到两种新的三萜糖苷（1 和 2）、17 种已知的三萜糖苷（3-19）、两种已知的黄酮苷（20 和 21）和两种已知的倍半萜葡萄糖苷（22 和 23）。通过包括二维 NMR 光谱在内的光谱分析以及水解产物的色谱分析确定了 1 和 2 的结构。评估了分离出的三萜糖苷（1-19）对 HL-60 人早幼粒细胞白血病细胞的细胞毒性。化合物 9、10 和 11 对 HL-60 细胞具有细胞毒性，IC 值分别为 7.2、21.9 和 32.8 μM。在 50 μM 的样品浓度下，从叶子中分离出的其他化合物没有细胞毒性。