Liu Chang, Zhao Ying, Xu Xiaoting, Zhang Lei, Cui Fengmei, Chen Qiu, Li Hongxia, Sang Ru, Li Gen, He Yongming
Department of Cardiology, First Affiliated Hospital of Soochow University, Suzhou, China.
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, China.
Dose Response. 2022 Jan 19;20(1):15593258211068649. doi: 10.1177/15593258211068649. eCollection 2022 Jan-Mar.
The aim is to explore the protective effects of Puerarin on radiation-induced vascular endothelial cell damage and its underlying mechanism. The apoptosis and DNA damage of Human umbilical vascular endothelial cells (HUVECs) exposed to radiation alone or in combination with glucose in the exposed group were significantly elevated ( < .05) compared with those in the control group. The Puerarin-treated HUVECs showed significant reduction in the radiation-induced apoptosis and DNA damage ( < .05). Furthermore, X-ray irradiation significantly increased the expression of miR-34a, which was reversed by pre-treatment with Puerarin. Placental Growth Factor (PLGF) was a target gene of miR-34a. The expression of PLGF in the peripheral blood of patients receiving radiotherapy significantly increased with an increase in the cumulative dose of radiation ( < .05), after which it began to decrease at the fourth week ( < .05) and then remained at a low level until the end of radiotherapy. Puerarin exerts a radioprotective effect by decreasing DNA damage and apoptosis through miR-34a-targeted PLGF.
目的是探讨葛根素对辐射诱导的血管内皮细胞损伤的保护作用及其潜在机制。与对照组相比，暴露组单独接受辐射或辐射联合葡萄糖处理的人脐静脉血管内皮细胞（HUVECs）的凋亡和DNA损伤显著升高（P<0.05）。经葛根素处理的HUVECs辐射诱导的凋亡和DNA损伤显著降低（P<0.05）。此外，X射线照射显著增加了miR-34a的表达，而葛根素预处理可使其逆转。胎盘生长因子（PLGF）是miR-34a的靶基因。接受放疗患者外周血中PLGF的表达随辐射累积剂量的增加而显著升高（P<0.05），之后在第四周开始下降（P<0.05），然后在放疗结束前一直维持在低水平。葛根素通过miR-34a靶向的PLGF减少DNA损伤和凋亡，从而发挥辐射保护作用。
