Rohilla Versha, Yadav Rajesh Kumar, Poonia Atman, Sheoran Ravika, Kumari Gita, Shanmugavadivel P S, Pratap Aditya
Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India.
ICAR-Indian Institute of Pulses Research, Kanpur, India.
Front Plant Sci. 2022 Jan 17;12:749439. doi: 10.3389/fpls.2021.749439. eCollection 2021.
Mung bean [ (L.) Wilczek] is an important short-duration grain legume widely known for its nutritional, soil ameliorative, and cropping system intensification properties. This study aims at evaluating genetic diversity among mung bean genotypes and detecting genomic regions associated with various yield attributing traits and yellow mosaic disease (YMD) resistance by association mapping. A panel of 80 cultivars and advanced breeding lines was evaluated for 10 yield-related and YMD resistance traits during (monsoon) and summer seasons of 2018-2019 and 2019-2020. A total of 164 genome-wide simple sequence repeat (SSR) markers were initially screened, out of which 89 were found polymorphic which generated 317 polymorphic alleles with an average of 3.56 alleles per SSR locus. The number of alleles at each locus varied from 2 to 7. The population genetic structure analysis grouped different genotypes in three major clusters and three genetically distinct subpopulations (SPs) (i.e., SP-1, SP-2, and SP-3) with one admixture subpopulation (SP-4). Both cluster and population genetic structure analysis categorized the advanced mung bean genotypes in a single group/SP and the released varieties in other groups/SPs, suggesting that the studied genotypes may have common ancestral history at some level. The population genetic structure was also in agreement with the genetic diversity analysis. The estimate of the average degree of linkage disequilibrium (LD) present at the genome level in 80 mung bean genotypes unveiled significant LD blocks. Over the four seasons, 10 marker-trait associations were observed significant for YMD and four seed yield (SY)-related traits ., days to flowering, days to maturity, plant height, and number of pods per plant using the mixed linear model (MLM) method. These associations may be useful for marker-assisted mung bean yield improvement programs and YMD resistance.
绿豆[ (L.) Wilczek]是一种重要的短生育期谷物豆类，以其营养、改良土壤和强化种植系统的特性而广为人知。本研究旨在通过关联分析评估绿豆基因型间的遗传多样性，并检测与各种产量相关性状和黄花叶病（YMD）抗性相关的基因组区域。在2018 - 2019年和2019 - 2020年的雨季和夏季，对一组80个品种和高级育种系进行了10个与产量相关和YMD抗性性状的评估。最初筛选了总共164个全基因组简单序列重复（SSR）标记，其中89个被发现具有多态性，产生了317个多态性等位基因，每个SSR位点平均有3.56个等位基因。每个位点的等位基因数量从2到7不等。群体遗传结构分析将不同基因型分为三个主要聚类和三个遗传上不同的亚群（即SP - 1、SP - 2和SP - 3）以及一个混合亚群（SP - 4）。聚类分析和群体遗传结构分析都将高级绿豆基因型归为一个单一组/亚群，而将已发布品种归为其他组/亚群，这表明所研究的基因型在某种程度上可能有共同的祖先历史。群体遗传结构也与遗传多样性分析一致。对80个绿豆基因型基因组水平上存在的连锁不平衡（LD）平均程度的估计揭示了显著的LD块。在四个季节中，使用混合线性模型（MLM）方法观察到10个标记 - 性状关联对于YMD和四个与种子产量（SY）相关的性状（即开花天数、成熟天数、株高和单株荚数）具有显著性。这些关联可能有助于绿豆产量改良计划中的标记辅助选择和YMD抗性研究。
