College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, Yunnan, China.
College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.
Arch Virol. 2022 Mar;167(3):959-963. doi: 10.1007/s00705-021-05269-7. Epub 2022 Feb 3.
The complete nucleotide sequence of a novel mycovirus, designated as "Rhizoctonia fumigata bipartite virus 1" (RfBV1), from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi was determined. The genome of RfBV1 is composed of two double-stranded RNAs (dsRNA). dsRNA-1 (2311 bp) contains one open reading frame (ORF), which codes for the putative RNA-dependent RNA polymerase (RdRp) of the virus. dsRNA-2 (1690 bp) contains one ORF, which encodes a putative protein whose function is unknown. Phylogenetic analysis indicated that the RdRp of RfBV1 clustered with several unassigned bipartite viruses belonging to the CThTV-like viruses group, but not the family Amalgaviridae or Partitiviridae. Our study suggests that RfBV1 is a novel mycovirus related to the CThTV-like viruses.
从 Rhizoctonia fumigata AG-Ba 分离株 C-314 Baishi 中,我们确定了一种新型真菌病毒的完整核苷酸序列,命名为“Rhizoctonia fumigata 二分体病毒 1”(RfBV1)。RfBV1 的基因组由两条双链 RNA(dsRNA)组成。dsRNA-1(2311 个碱基对)包含一个开放阅读框(ORF),编码病毒的 RNA 依赖性 RNA 聚合酶(RdRp)。dsRNA-2(1690 个碱基对)包含一个 ORF,编码一种未知功能的假定蛋白。系统发育分析表明,RfBV1 的 RdRp 与属于 CThTV 样病毒群的几个未分类的二分体病毒聚类,但不属于 Amalgaviridae 或 Partitiviridae 家族。我们的研究表明,RfBV1 是一种与 CThTV 样病毒相关的新型真菌病毒。