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一株引起立枯丝核菌 AG-Ba 分离物 C-314 白石病的新型真菌病毒的全核苷酸序列。

Complete nucleotide sequence of a novel mycovirus infecting Rhizoctonia fumigata AG-Ba isolate C-314 Baishi.

机构信息

College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, Yunnan, China.

College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.

出版信息

Arch Virol. 2022 Mar;167(3):959-963. doi: 10.1007/s00705-021-05269-7. Epub 2022 Feb 3.

Abstract

The complete nucleotide sequence of a novel mycovirus, designated as "Rhizoctonia fumigata bipartite virus 1" (RfBV1), from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi was determined. The genome of RfBV1 is composed of two double-stranded RNAs (dsRNA). dsRNA-1 (2311 bp) contains one open reading frame (ORF), which codes for the putative RNA-dependent RNA polymerase (RdRp) of the virus. dsRNA-2 (1690 bp) contains one ORF, which encodes a putative protein whose function is unknown. Phylogenetic analysis indicated that the RdRp of RfBV1 clustered with several unassigned bipartite viruses belonging to the CThTV-like viruses group, but not the family Amalgaviridae or Partitiviridae. Our study suggests that RfBV1 is a novel mycovirus related to the CThTV-like viruses.

摘要

从 Rhizoctonia fumigata AG-Ba 分离株 C-314 Baishi 中,我们确定了一种新型真菌病毒的完整核苷酸序列,命名为“Rhizoctonia fumigata 二分体病毒 1”(RfBV1)。RfBV1 的基因组由两条双链 RNA(dsRNA)组成。dsRNA-1(2311 个碱基对)包含一个开放阅读框(ORF),编码病毒的 RNA 依赖性 RNA 聚合酶(RdRp)。dsRNA-2(1690 个碱基对)包含一个 ORF,编码一种未知功能的假定蛋白。系统发育分析表明,RfBV1 的 RdRp 与属于 CThTV 样病毒群的几个未分类的二分体病毒聚类,但不属于 Amalgaviridae 或 Partitiviridae 家族。我们的研究表明,RfBV1 是一种与 CThTV 样病毒相关的新型真菌病毒。

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