College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, Yunnan, China.
Yunnan Agricultural University, Kunming, 650201, Yunnan, China.
Arch Virol. 2022 Mar;167(3):953-957. doi: 10.1007/s00705-021-05261-1. Epub 2022 Feb 3.
In this report, the full genome sequence of a novel mycovirus, designated as "Rhizoctonia solani partitivirus SM03" (RsPV-SM03), was determined in Rhizoctonia solani AG-4 HG III isolate SM03. RsPV-SM03 genome consists of two dsRNAs (dsRNA-1 and dsRNA-2), each of them contains one single open reading frame (ORF). ORF1 of dsRNA-1 encodes a putative RNA-dependent RNA polymerase (RdRp), while ORF2 of dsRNA-2 encodes a putative viral coat protein (CP). Phylogenetic analysis indicated that the RdRp and CP of RsPV-SM03 are closely related to those of other members of the genus Alphapartitivirus, family Partitiviridae, suggesting that RsPV-SM03 represents a novel species in the genus Alphapartitivirus.
在本报告中,我们确定了一株新型真菌病毒——“Rhizoctonia solani partitivirus SM03”(RsPV-SM03)的全基因组序列。该病毒源自 Rhizoctonia solani AG-4 HG III 分离株 SM03。RsPV-SM03 基因组由两条 dsRNA(dsRNA-1 和 dsRNA-2)组成,每条 dsRNA 包含一个单一的开放阅读框(ORF)。dsRNA-1 的 ORF1 编码一个假定的 RNA 依赖性 RNA 聚合酶(RdRp),而 dsRNA-2 的 ORF2 编码一个假定的病毒外壳蛋白(CP)。系统发育分析表明,RsPV-SM03 的 RdRp 和 CP 与其他 Alpha partitivirus 属成员的 RdRp 和 CP 密切相关,这表明 RsPV-SM03 代表了 Alpha partitivirus 属中的一个新种。