College of Plant Protection, China Agricultural University, Haidian District, 100193, Beijing, People's Republic of China.
Arch Virol. 2023 Sep 5;168(10):241. doi: 10.1007/s00705-023-05868-6.
A novel mycovirus, Ceratobasidium bipartite virus 1 (CBV1), was identified in Ceratobasidium sp. AG-A strain SHX-YJLC-1 isolated from diseased potato stems. The complete genome of CBV1 consists of two double-stranded RNA (dsRNA) segments: dsRNA1 (2311 bp) and dsRNA2 (1761 bp). dsRNA1 contains a single open reading frame (ORF1) encoding an RNA-dependent RNA polymerase (RdRp), while dsRNA2 contains a single ORF (ORF2) encoding a hypothetical protein (HP) with unknown function. BLASTp analysis revealed that RdRp (75.04%) and HP (61.86%) encoded by the two ORFs have the highest sequence similarity to their counterparts in Rhizoctonia solani dsRNA virus 11 (RsRV11). The genome organization and phylogenetic analysis indicated that the closest relatives to CBV1 are members of the proposed family "Bipartitiviridae". Based on the collective results, CBV1 is inferred to be a new member of the proposed family "Bipartitiviridae". This is the first report on the complete genome sequence of the novel bipartitivirus CBV1, which infects Ceratobasidium sp. AG-A strain SHX-YJLC-1.
一种新型的真菌病毒，即 Ceratobasidium bipartite virus 1（CBV1），在从患病马铃薯茎中分离出的 Ceratobasidium sp. AG-A 菌株 SHX-YJLC-1 中被鉴定出来。CBV1 的完整基因组由两条双链 RNA（dsRNA）片段组成：dsRNA1（2311 bp）和 dsRNA2（1761 bp）。dsRNA1 包含一个单一的开放阅读框（ORF1），编码 RNA 依赖性 RNA 聚合酶（RdRp），而 dsRNA2 包含一个单一的 ORF（ORF2），编码一个具有未知功能的假定蛋白（HP）。BLASTp 分析表明，两个 ORF 编码的 RdRp（75.04%）和 HP（61.86%）与 Rhizoctonia solani dsRNA virus 11（RsRV11）中的相应蛋白具有最高的序列相似性。基因组组织和系统发育分析表明，与 CBV1 最接近的亲缘关系是拟议的“Bipartitiviridae”家族的成员。基于这些综合结果，推断 CBV1 是拟议的“Bipartitiviridae”家族的一个新成员。这是首例关于感染 Ceratobasidium sp. AG-A 菌株 SHX-YJLC-1 的新型二分体病毒 CBV1 的完整基因组序列的报告。
