State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Arch Virol. 2022 Mar;167(3):983-987. doi: 10.1007/s00705-022-05360-7. Epub 2022 Feb 3.
A novel wheat-infecting polerovirus, tentatively named "wheat yellow dwarf virus" (WYDV), was identified from winter wheat in China using transcriptome sequencing (RNA-seq) combined with RT-PCR and RACE amplification. WYDV has a single-stranded RNA genome of 5,650 nucleotides (nt) and contains seven putative open reading frames (ORFs). WYDV was found to share the highest sequence identity with cereal yellow dwarf virus RPV (CYDV-RPV, genus Polerovirus), 71.1% for the nucleotide sequence of the whole genome, and 77.3% and 70.0% for the amino acid sequences of the coat protein (CP) and RNA-dependent RNA polymerase protein (RdRp), respectively. Phylogenetic analysis based on the complete genome and CP and RdRp amino acid sequences showed that WYDV is most closely related to the cereal-infecting poleroviruses CYDV-RPV, CYDV-RPS, and barley yellow dwarf virus-GPV. These data suggest that WYDV, which is associated with a newly emerging yellow dwarf disease in wheat fields in central China, should be classified as a new member of the genus Polerovirus.
一种新的侵染小麦的杆状病毒，暂命名为“小麦黄矮病毒”（WYDV），在中国的冬小麦中通过转录组测序（RNA-seq）结合 RT-PCR 和 RACE 扩增被鉴定出来。WYDV 的基因组为 5650 个核苷酸（nt）的单链 RNA，包含七个推定的开放阅读框（ORFs）。WYDV 与禾谷多黏菌病毒 RPV（CYDV-RPV，杆状病毒属）的序列具有最高的同源性，全基因组的核苷酸序列为 71.1%，外壳蛋白（CP）和 RNA 依赖性 RNA 聚合酶蛋白（RdRp）的氨基酸序列分别为 77.3%和 70.0%。基于全基因组和 CP 和 RdRp 氨基酸序列的系统发育分析表明，WYDV 与侵染禾本科的杆状病毒 CYDV-RPV、CYDV-RPS 和大麦黄矮病毒-GPV 最为密切相关。这些数据表明，与中国中部麦田新兴的黄矮病相关的 WYDV 应被归类为杆状病毒属的一个新成员。
