Zhou Zhiguo, Feng Xue, Liang Linzhu, Liu Wenya, Zhou Zixuan, Zhang Xinyuan, Xu Zhongtian, Zhang Peipei
College of Life Sciences, Langfang Normal University, Langfang, Hebei, 065000, China.
Institute of Plant Virology, Ningbo University, Ningbo, Zhejiang, 315211, China.
Arch Virol. 2025 Jun 2;170(7):145. doi: 10.1007/s00705-025-06336-z.
To determine the prevalence of wheat-infecting viruses, a viromic survey of wheat plants exhibiting typical plant virus symptoms collected in Hebei province, China, was conducted using rRNA-depleted RNA-seq. A novel luteovirus was discovered and tentatively named "wheat luteovirus 1" (WLV1). RT-PCR and RACE-PCR were performed to determine the complete genome sequence of WLV1, which was found to consist of 5,682 nucleotides (nt), with six AUG-initiated ORFs and one non-AUG-initiated ORF, and to have typical characteristics of members of the genus Luteovirus. Sequence comparisons and phylogenetic analysis indicated that WLV1 was most closely related to several barley yellow dwarf viruses (BYDVs) belonging to the genus Luteovirus, but the level of sequence identity was below the species demarcation threshold (90%). This finding therefore adds a new luteovirus to the list of viruses associated with wheat yellow dwarf disease.
为了确定感染小麦的病毒的流行情况，利用去除rRNA的RNA测序技术，对在中国河北省采集的表现出典型植物病毒症状的小麦植株进行了病毒组调查。发现了一种新型黄症病毒，并暂时命名为“小麦黄症病毒1”（WLV1）。进行了RT-PCR和RACE-PCR以确定WLV1的完整基因组序列，发现其由5682个核苷酸（nt）组成，有六个AUG起始的开放阅读框（ORF）和一个非AUG起始的ORF，并且具有黄症病毒属成员的典型特征。序列比较和系统发育分析表明，WLV1与黄症病毒属的几种大麦黄矮病毒（BYDVs）关系最为密切，但序列同一性水平低于种的划分阈值（90%）。因此，这一发现为与小麦黄矮病相关的病毒列表增添了一种新的黄症病毒。
