School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA.
USDA-ARS Subtropical Horticultural Research Station, Miami, FL, 33158, USA.
Arch Virol. 2024 Mar 23;169(4):83. doi: 10.1007/s00705-024-06013-7.
The complete genome sequence of cacao leafroll virus (CaLRV; family Solemoviridae, genus Polerovirus) was determined by high-throughput sequencing of total RNA isolated from symptomatic cacao Theobroma cacao L. plants (n = 4). The CaLRV genome sequences ranged from 5,976 to 5,997 nucleotides (nt) in length and contained seven open reading frames (ORFs). Nucleotide and amino acid (aa) sequence comparisons showed that, among selected well-characterized poleroviruses, the CaLRV genome shared the highest nt sequence identity of 62% with that of potato leafroll virus (PLRV, NC_076505). A comparison of the predicted aa sequence of the CaLRV coat protein indicated that cotton leafroll dwarf virus (CLRDV, NC_014545) and melon aphid-borne yellows virus (MABYV, NC_010809) were the closest relatives, sharing 57% aa sequence identity. Bayesian phylogenetic analysis based on complete genome sequences showed that CaLRV grouped with well-characterized poleroviruses that cause diseases of cereal and vegetable crops. During the course of publishing this work, the nearly complete genome sequence of a member of the same polerovirus species, referred to as "cacao polerovirus" (OR605721), with which CaLRV shares 99% nt sequence identity, was reported.
可可卷叶病毒（CaLRV；Solemoviridae 科，Polerovirus 属）的全基因组序列通过高通量测序从感染症状的可可 Theobroma cacao L. 植物（n=4）中分离的总 RNA 确定。CaLRV 基因组序列长度从 5976 到 5997 个核苷酸（nt）不等，包含七个开放阅读框（ORF）。核苷酸和氨基酸（aa）序列比较表明，在选定的特征明确的杆状病毒中，CaLRV 基因组与马铃薯卷叶病毒（PLRV，NC_076505）的核苷酸序列同源性最高，为 62%。对 CaLRV 外壳蛋白预测 aa 序列的比较表明，棉花卷叶矮缩病毒（CLRDV，NC_014545）和瓜蚜传黄化病毒（MABYV，NC_010809）是最接近的亲缘病毒，共享 57%的 aa 序列同一性。基于完整基因组序列的贝叶斯系统发育分析表明，CaLRV 与引起谷类和蔬菜作物疾病的特征明确的杆状病毒聚为一组。在发表这项工作的过程中，报道了同一杆状病毒种的一个成员的近乎完整的基因组序列，称为“可可杆状病毒”（OR605721），与 CaLRV 共享 99%的 nt 序列同一性。
