Department of Pediatric Surgery, National Pirogov Memorial Medical University, Vinnytsya, Ukraine.
Pediatr Endocrinol Diabetes Metab. 2022;28(1):30-34. doi: 10.5114/pedm.2022.112497.
Impact of the secondary peritoneal infections on the peritoneal morphology in diabetic population remains unclear.
To study the histopathological changes of the peritoneum due to secondary bacterial peritonitis in diabetic rats in compari-son to the normoglycemic animals.
13 adult male Wistar rats were divided into 2 groups: control (n = 3) and experimental (n = 10). The latter was subdivided into non-diabetic (n = 5) and diabetic (n = 5). T1DM and peritonitis were induced through the single intraperitoneal injec-tion of Streptozotocin (60 mg/kg) and intraperitoneal injection of 2 ml of the faecal matter, respectively.
Macroscopic findings of diabetic rat peritoneum included significant accumulation of the exudate in the peritoneal cavity and presence of intraintestinal adhesions and abscesses. Morphology of the peritonium presented with diffuse, profound degenerative and inflammatory lesions involving the subjacent tissues. In contrast, the underlying muscular layer of the non-diabetic rats remained intact.
Natural course of the peritoneal infection in diabetic rats (48 hours) is characterised by diffuse severe peritonitis with inter-intestinal abscess formation and more profound subadjacent tissue involvement in comparison with non-diabetic rats.
糖尿病患者发生继发性腹膜感染对腹膜形态的影响尚不清楚。
研究继发细菌性腹膜炎对糖尿病大鼠腹膜的组织病理学变化，并与正常血糖动物进行比较。
将 13 只成年雄性 Wistar 大鼠分为 2 组：对照组（n = 3）和实验组（n = 10）。后者进一步分为非糖尿病组（n = 5）和糖尿病组（n = 5）。通过单次腹腔注射链脲佐菌素（60mg/kg）和腹腔内注射 2ml 粪便，分别诱导 1 型糖尿病和腹膜炎。
糖尿病大鼠腹膜的大体观察结果包括腹腔内渗出物明显积聚，存在肠粘连和脓肿。腹膜形态表现为弥漫性、严重的退行性和炎症性病变，累及下组织。相比之下，非糖尿病大鼠的下肌层保持完整。
糖尿病大鼠（48 小时）腹膜感染的自然病程表现为弥漫性严重腹膜炎，伴有肠间脓肿形成，下组织受累更严重，与非糖尿病大鼠相比。
