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利用超滤酶制剂从甘蔗渣中生产更高质量纸张的环保生物催化工艺。

An eco-friendly biocatalytic process for producing better quality paper from sugarcane bagasse using ultrafiltered enzymes concoction.

机构信息

Department of Biotechnology, Kurukshetra University, Kurukshetra, India.

Department of Life Sciences, Sharda University, Greater Noida, India.

出版信息

Bioprocess Biosyst Eng. 2022 Apr;45(4):741-747. doi: 10.1007/s00449-022-02695-y. Epub 2022 Feb 3.

Abstract

In the current study, pretreatment of sugarcane bagasse has been carried out with ultrafiltered xylano-pectinolytic enzymes, before conventional chemical bleaching process. Optimized enzymatic dose (4 IU xylanase and 1.2 IU pectinase per g of oven dried pulp) and retention time (180 min) were determined on the basis of maximum decrement in kappa number (from 20.93 to 15.32), release of maximum sugars (7.4 mg/g) as well as attainment of maximum brightness (25.1% ISO), whiteness (from - 57.3 to - 41.9) and minimum yellowness (from 48.7 to 35.3) of the pulp samples. Enzymatically treated samples also showed release of phenolic, lignin and hydrophobic compounds in their filtrates. Nearly 30% decrement in the exhaustion of bleaching chemical dose was detected as compared to control samples. The physical properties such as tear index, burst index, double fold number, breaking length, gurley porosity and viscosity of enzymo-chemically treated bagasse pulp samples were improved by 6.68%, 33.86%, 22.92%, 13.43%, 17.5% and 9.64%, respectively. Additionally, a decrement of 36.75% and 28.29% in the values of BOD and COD of the effluents was also noted, which demonstrated the fact that, inclusion of enzymes in chemical based protocols of paper and pulp industries could be a highly beneficial and eco-friendly approach in upcoming decades. This is the first report mentioning the effect of ultrafiltered xylano-pectinolytic enzymes concoction on sugarcane bagasse pulp.

摘要

在本研究中,在常规化学漂白工艺之前,用超滤木聚糖-果胶酶对甘蔗渣进行了预处理。根据最大卡伯值下降(从 20.93 降至 15.32)、最大糖释放(7.4mg/g)以及最大亮度(25.1%ISO)、白度(从-57.3 降至-41.9)和最小黄度(从 48.7 降至 35.3)达到最佳值,确定了最佳酶剂量(4IU 木聚糖酶和 1.2IU 果胶酶/每克干浆)和保留时间(180 分钟)。酶处理后的样品也显示出其滤液中释放出酚类、木质素和疏水性化合物。与对照样品相比,漂白化学剂量的消耗减少了近 30%。酶化学处理的蔗渣浆样品的物理性能如撕裂指数、耐破指数、双折数、断裂长度、古尔里透气度和粘度分别提高了 6.68%、33.86%、22.92%、13.43%、17.5%和 9.64%。此外,还注意到废水的 BOD 和 COD 值分别降低了 36.75%和 28.29%,这表明在未来几十年,将酶纳入纸浆和造纸工业的基于化学的方案中可能是一种非常有益和环保的方法。这是首次报道超滤木聚糖-果胶酶混合物对甘蔗渣浆的影响。

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