Sharma Divya, Agrawal Sharad, Nagpal Raksha, Mishra O P, Bhardwaj Nishikant, Mahajan Ritu
Department of Biotechnology, Kurukshetra University, Kurukshetra, India.
Department of Life Sciences, IAMR College, Ghaziabad, India.
3 Biotech. 2023 Feb;13(2):61. doi: 10.1007/s13205-023-03484-4. Epub 2023 Jan 26.
This research aimed to investigate the efficiency of crude xylanase-pectinase in pulping of sugarcane bagasse. Optimum biopulping was obtained, using xylanase-pectinase dose 200-60 IU/g, bagasse/liquid ratio 1:10 and 1.0% Tween 80 concentration at 55 °C temperature, pH 8.5 and period of treatment 180 min. Treatment of sugarcane bagasse samples with these enzymes generated pulp with lower rejections (58.76%), total solids (12.64%), kappa number (47.77%), higher screened pulp yield (10.66%), along with enhanced optical and physical properties, in comparison with a chemical pulp. Bagasse biopulping resulted in a 13% decrease in alkali dose to obtain the optical and physical properties similar to those achieved under the 100% alkali dose. The breaking length, burst factor, tear index, double fold, gurley porosity and viscosity were improved by 15.19, 37.64, 2.47, 37.77, 35 and 23.17%, respectively, after bleaching treatment of biopulped samples. Thus, enzymatic pulping is an eco-friendly environmentally sustainable approach, since it reduces the use of pulping chemicals and simultaneously improves the paper quality. This is the first report, showing pulping of sugarcane bagasse, with crude xylanase-pectinase, produced by an isolate.
本研究旨在探究粗木聚糖酶 - 果胶酶用于甘蔗渣制浆的效率。在温度55℃、pH值8.5、处理时间180分钟的条件下,使用200 - 60 IU/g的木聚糖酶 - 果胶酶剂量、1:10的蔗渣/液体比例和1.0%的吐温80浓度,可获得最佳生物制浆效果。与化学浆相比,用这些酶处理甘蔗渣样品所产生的纸浆具有更低的筛渣率(58.76%)、总固形物含量(12.64%)、卡伯值(47.77%),更高的筛选纸浆得率(10.66%),以及增强的光学和物理性能。蔗渣生物制浆可使碱用量减少13%,以获得与100%碱用量下相似的光学和物理性能。对生物制浆样品进行漂白处理后,其抗张强度、破裂因子、撕裂指数、耐折度、格利透气度和粘度分别提高了15.19%、37.64%、2.47%、37.77%、35%和23.17%。因此,酶法制浆是一种环保且可持续的方法,因为它减少了制浆化学品的使用,同时提高了纸张质量。这是首次报道用分离菌株产生的粗木聚糖酶 - 果胶酶对甘蔗渣进行制浆。