从正常胎儿皮肤成纤维细胞建立人诱导多能干细胞系MUi028-A。
Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts.
作者信息
Chumchuen Sukanya, Satirapod Chonthicha, Tangprasittipap Amornrat, Hongeng Suradej
机构信息
Office of Research, Academic Affairs and Innovations, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
Department of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
出版信息
Stem Cell Res. 2022 Apr;60:102675. doi: 10.1016/j.scr.2022.102675. Epub 2022 Jan 19.
MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A cell line had embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a control to study disease mechanisms.
MUi028-A人诱导多能干细胞系是通过非整合重编程方法从正常胎儿皮肤成纤维细胞中产生的。通过电穿孔法将三个附加型载体中的重编程因子OCT4、SOX2、KLF4、L-MYC和LIN28以及TP53 shRNA导入细胞。MUi028-A细胞系具有胚胎干细胞特征，特定多能性标志物表达一致，且具有体外分化为三个胚层的能力。该诱导多能干细胞系可作为研究疾病机制的对照。
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