从纯合α地中海贫血胎儿皮肤成纤维细胞中生成诱导多能干细胞系MU011.A-hiPS
Generation of iPSC line MU011.A-hiPS from homozygous α-thalassemia fetal skin fibroblasts.
作者信息
Tangprasittipap Amornrat, Satirapod Chonthicha, Jittorntrum Bunyada, Lertritanan Sassawat, Anurathaphan Usanarat, Phanthong Phetcharat, Borwornpinyo Suparerk, Kitiyanant Narisorn, Hongeng Suradej
机构信息
Office of Research, Academic Affairs and Innovations, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Department of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
出版信息
Stem Cell Res. 2015 Nov;15(3):506-9. doi: 10.1016/j.scr.2015.09.003. Epub 2015 Sep 21.
Human iPSC line MU011.A-hiPS was generated from homozygous α-thalassemia (-(SEA)/-(SEA)) fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT3/4, SOX2, KLF4, L-MYC, LIN28, and shRNA of TP53 contained in three episomal vectors were delivered using electroporation.
人诱导多能干细胞系MU011.A-hiPS是使用非整合重编程方法从纯合α地中海贫血（-(SEA)/-(SEA)）胎儿皮肤成纤维细胞中产生的。通过电穿孔递送包含在三个游离载体中的重编程因子OCT3/4、SOX2、KLF4、L-MYC、LIN28和TP53的短发夹RNA。
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