Krittika Sudhakar, Yadav Pankaj
Fly Laboratory no. 210, Anusandhan Kendra-II, School of Chemical & Biotechnology, SASTRA Deemed to be University, Thanjavur 613401, Tamil Nadu, India.
R Soc Open Sci. 2022 Jan 26;9(1):211325. doi: 10.1098/rsos.211325. eCollection 2022 Jan.
Protein restriction (PR) has established feasible trade-offs in to understand lifespan or ageing in a nutritionally challenged environment. However, the phenotypes of body size, weight and wing length respond according to factors such as flies' genotype, environmental exposure and parental diet, and hence their understanding is essential. Here, we demonstrate the effect of long-term PR diet on body size, weight, normal and dry wing length of flies subjected to PR50 and PR70 (50% and 70% protein content present in control food, respectively) for 20 generations from the pre-adult stage. We found that PR-fed flies have lower body weight, relative water content (in males), unaltered (PR50%) and higher (PR70%) relative fat content in males, smaller normal and dry body size when compared with control and generations 1 and 2. Interestingly, the wing size and pupal size of PR flies are smaller and showed significant effects on diet and generation. Thus, these traits are sex and generation dependent along with a diet interaction, which is capable of modulating these results variably. Taken together, the trans-generational effect of PR on fitness and fitness-related traits might be helpful to understand the underpinning mechanisms of evolution and ageing in fruit flies .
蛋白质限制（PR）已在营养挑战环境中建立了可行的权衡，以了解寿命或衰老。然而，体型、体重和翅长的表型会根据果蝇的基因型、环境暴露和亲代饮食等因素做出反应，因此对它们的理解至关重要。在这里，我们展示了长期PR饮食对从成虫前期开始接受PR50和PR70（分别为对照食物中蛋白质含量的50%和70%）处理20代的果蝇的体型、体重、正常翅长和干翅长的影响。我们发现，与对照组以及第1代和第2代相比，接受PR喂养的果蝇体重较低，雄性相对含水量较低（雄性），雄性相对脂肪含量不变（PR50%）且较高（PR70%），正常和干体型较小。有趣的是，PR果蝇的翅尺寸和蛹尺寸较小，并且对饮食和代数有显著影响。因此，这些性状具有性别和代数依赖性以及饮食相互作用，这能够以不同方式调节这些结果。综上所述，PR对适应性和适应性相关性状的跨代效应可能有助于理解果蝇进化和衰老的潜在机制。
