Krittika Sudhakar, Yadav Pankaj
Fly Laboratory # 210, Anusandhan Kendra-II, School of Chemical & Biotechnology, SASTRA Deemed to be University, Thanjavur, Tamil Nadu 613401, India.
R Soc Open Sci. 2024 Jun 5;11(6):231741. doi: 10.1098/rsos.231741. eCollection 2024 Jun.
Studies in fruit flies, , have observed considerable variation in the effect of dietary protein restriction (PR) on various fitness traits. In addition, not only are there inconsistent results relating lifespan to stress resistance, but also the long-term effects of PR are unexplored. We study PR implementation across generations (long term) hypothesizing that it will be beneficial for fitness traits, stress resistance and storage reserves due to nutritional plasticity transferred by parents to offspring in earlier studies. By imposing two concentrations of PR diets (50% and 70% of control protein) from the pre-adult and adult (age 1 day) stages of the flies, we assessed the stage-specific and long-term effect of the imposed PR. All long-term PR flies showed increased resistance against the tested stressors (starvation, desiccation, HO-induced oxidative stress). In addition, we also found long-term PR-induced increased stress resistance across generations. The PR flies also possessed higher protein and triglyceride (TG) content, reduced glucose and unaffected glycogen levels. We also assayed the effect of returning the PR flies to control (AL) food for a single generation and assessed their biochemical parameters to witness the transient PR effect. It was seen that TG content upon reversal was similar to AL flies except for PRI70 males; however, the glucose levels of PR males increased, while they were consistently lower in females. Taken altogether, our study suggests that long-term PR implementation contributes to increased stress resistance and was found to influence storage reserves in .
对果蝇的研究已经观察到，饮食蛋白质限制（PR）对各种健康性状的影响存在相当大的差异。此外，不仅寿命与抗逆性之间的结果不一致，而且PR的长期影响也未得到探索。我们研究了跨代（长期）实施PR的情况，假设由于在早期研究中父母传递给后代的营养可塑性，这将有利于健康性状、抗逆性和储存储备。通过在果蝇的成虫前期和成虫期（1日龄）施加两种浓度的PR饮食（对照蛋白质的50%和70%），我们评估了施加的PR的阶段特异性和长期影响。所有长期接受PR的果蝇对测试应激源（饥饿、干燥、过氧化氢诱导的氧化应激）的抵抗力都有所增强。此外，我们还发现长期PR诱导的跨代抗逆性增加。接受PR的果蝇还具有较高的蛋白质和甘油三酯（TG）含量，葡萄糖含量降低，糖原水平未受影响。我们还检测了将接受PR的果蝇恢复到对照（正常饮食，AL）食物一代后的影响，并评估了它们的生化参数，以观察短暂的PR效应。可以看到，除了PRI70雄性果蝇外，逆转后的TG含量与AL果蝇相似；然而，PR雄性果蝇的葡萄糖水平升高，而雌性果蝇的葡萄糖水平一直较低。总的来说，我们的研究表明，长期实施PR有助于增强抗逆性，并且发现其会影响果蝇的储存储备。
