• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

THZ1处理的鼻咽癌细胞系的基因表达谱表明其参与细胞周期抑制。

Gene expression profile of THZ1-treated nasopharyngeal carcinoma cell lines indicates its involvement in the inhibition of the cell cycle.

作者信息

Gao Lijuan, Xia Shuang, Zhang Kunyi, Lin Chengguang, He Xuyu, Zhang Ying

机构信息

Sun Yat-sen University Cancer Center, Sun Yat-sen University, Guangzhou, China.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University, Guangzhou, China.

出版信息

Transl Cancer Res. 2021 Jan;10(1):445-460. doi: 10.21037/tcr-19-2888.

DOI:10.21037/tcr-19-2888
PMID:35116274
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8799269/
Abstract

BACKGROUND

The aim of this study was to identify downstream target genes and pathways regulated by THZ1 in nasopharyngeal carcinoma (NPC).

METHODS

The gene expression profile of GSE95750 in two NPC cell lines, untreated group and treated with THZ1 group, was analyzed. Differentially expressed genes (DEGs) were compared using the R-software. Then Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathways (KEGG) was analyzed using Database for Annotation, Visualization, and Integrated Discovery (DAVID). Cytoscape was used for protein-protein interaction (PPI) analysis. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to verified the gene expression.

RESULTS

We identified 25 genes with increased expression and 567 genes with decreased expression in THZ1-treated NPC cells. The top 10 significantly DEGs between untreated group and THZ1 treated group were identified by qRT-PCR and the results were in agreement with RNA-seq. The total 592 DEGs were found enriched in 1,148 GO terms and 38 KEGG pathways. The most important enriched pathways identified were cell cycle related, and several related node genes were identified, such as , , , , , , , , , , and family, which consistent with RNA-seq.

CONCLUSIONS

Our results emphasize the differential genes and pathways occurring in THZ1-treated NPC cells, which increases our understanding of the anti-tumor mechanisms of THZ1.

摘要

背景

本研究旨在鉴定噻唑烷二酮类化合物1（THZ1）在鼻咽癌（NPC）中调控的下游靶基因和信号通路。

方法

分析了GSE95750在两种NPC细胞系（未处理组和THZ1处理组）中的基因表达谱。使用R软件比较差异表达基因（DEG）。然后使用注释、可视化和综合发现数据库（DAVID）分析基因本体（GO）和京都基因与基因组百科全书通路（KEGG）。使用Cytoscape进行蛋白质-蛋白质相互作用（PPI）分析。采用定量逆转录聚合酶链反应（qRT-PCR）验证基因表达。

结果

我们在THZ1处理的NPC细胞中鉴定出25个表达增加的基因和567个表达降低的基因。通过qRT-PCR鉴定了未处理组和THZ1处理组之间前10个显著差异表达基因，结果与RNA测序一致。总共592个差异表达基因在1148个GO术语和38条KEGG通路中富集。鉴定出的最重要的富集通路与细胞周期相关，并鉴定了几个相关的节点基因，如 、 、 、 、 、 、 、 、 、 、 家族，这与RNA测序结果一致。

结论

我们的结果强调了THZ1处理的NPC细胞中出现的差异基因和信号通路，这增加了我们对THZ1抗肿瘤机制的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/1e0e5354c2b8/tcr-10-01-445-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/01c6ea899385/tcr-10-01-445-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/8e9d3e691033/tcr-10-01-445-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/eb6b278889c5/tcr-10-01-445-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/7f1634b93b42/tcr-10-01-445-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/873286707c9c/tcr-10-01-445-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/a0c1a5c7acc4/tcr-10-01-445-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/9478d81f64f9/tcr-10-01-445-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/1e0e5354c2b8/tcr-10-01-445-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/01c6ea899385/tcr-10-01-445-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/8e9d3e691033/tcr-10-01-445-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/eb6b278889c5/tcr-10-01-445-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/7f1634b93b42/tcr-10-01-445-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/873286707c9c/tcr-10-01-445-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/a0c1a5c7acc4/tcr-10-01-445-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/9478d81f64f9/tcr-10-01-445-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca69/8799269/1e0e5354c2b8/tcr-10-01-445-f8.jpg

相似文献

1
Gene expression profile of THZ1-treated nasopharyngeal carcinoma cell lines indicates its involvement in the inhibition of the cell cycle.THZ1处理的鼻咽癌细胞系的基因表达谱表明其参与细胞周期抑制。
Transl Cancer Res. 2021 Jan;10(1):445-460. doi: 10.21037/tcr-19-2888.
2
Transcriptome profile and its partly verification of human hepatocellular carcinoma cells exposed to Yuzhizi () seed extract.转录组谱及其对人肝癌细胞暴露于郁李仁种子提取物的部分验证。
J Tradit Chin Med. 2022 Dec;42(6):922-931. doi: 10.19852/j.cnki.jtcm.20220817.002.
3
Investigation of differentially expressed genes in nasopharyngeal carcinoma by integrated bioinformatics analysis.通过综合生物信息学分析研究鼻咽癌中差异表达基因
Oncol Lett. 2019 Jul;18(1):916-926. doi: 10.3892/ol.2019.10382. Epub 2019 May 21.
4
Bioinformatics Analysis of Candidate Genes and Pathways Related to Hepatocellular Carcinoma in China: A Study Based on Public Databases.中国肝细胞癌相关候选基因和通路的生物信息学分析：基于公共数据库的研究。
Pathol Oncol Res. 2021 Mar 26;27:588532. doi: 10.3389/pore.2021.588532. eCollection 2021.
5
Identification of key pathways and genes in nasopharyngeal carcinoma using bioinformatics analysis.利用生物信息学分析鉴定鼻咽癌中的关键通路和基因。
Oncol Lett. 2019 May;17(5):4683-4694. doi: 10.3892/ol.2019.10133. Epub 2019 Mar 8.
6
Differentially Expressed Genes in Nasopharyngeal Carcinoma Tissues and Their Correlation with Recurrence and Metastasis of Nasopharyngeal Carcinoma.鼻咽癌组织中差异表达的基因及其与鼻咽癌复发转移的关系。
Comput Math Methods Med. 2022 Apr 25;2022:1941412. doi: 10.1155/2022/1941412. eCollection 2022.
7
A Novel Hypoxia-Related Gene Signature with Strong Predicting Ability in Non-Small-Cell Lung Cancer Identified by Comprehensive Profiling.通过综合分析鉴定出一种在非小细胞肺癌中具有强大预测能力的新型缺氧相关基因特征。
Int J Genomics. 2022 May 19;2022:8594658. doi: 10.1155/2022/8594658. eCollection 2022.
8
[Bioinformatics analysis of core differentially expressed genes in hepatitis B virus-related hepatocellular carcinoma].[乙型肝炎病毒相关肝细胞癌核心差异表达基因的生物信息学分析]
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2022 Nov 21;34(5):507-513. doi: 10.16250/j.32.1374.2021292.
9
Bioinformatic identification of candidate biomarkers and related transcription factors in nasopharyngeal carcinoma.鼻咽癌候选生物标志物及相关转录因子的生物信息学鉴定。
World J Surg Oncol. 2019 Apr 1;17(1):60. doi: 10.1186/s12957-019-1605-9.
10
Screening Key Genes and Biological Pathways in Nasopharyngeal Carcinoma by Integrated Bioinformatics Analysis.基于整合生物信息学分析的鼻咽癌关键基因和生物学通路筛查。
Int J Mol Sci. 2022 Dec 11;23(24):15701. doi: 10.3390/ijms232415701.

引用本文的文献

1
Switch-like gene expression modulates disease risk.开关式基因表达调节疾病风险。
Nat Commun. 2025 Jun 18;16(1):5323. doi: 10.1038/s41467-025-60513-x.
2
Switch-like Gene Expression Modulates Disease Susceptibility.开关式基因表达调节疾病易感性。
Res Sq. 2024 Sep 13:rs.3.rs-4974188. doi: 10.21203/rs.3.rs-4974188/v1.
3
Switch-like Gene Expression Modulates Disease Susceptibility.开关式基因表达调节疾病易感性。

本文引用的文献

1
Identification of novel biomarkers and small-molecule compounds for nasopharyngeal carcinoma with metastasis.鼻咽癌转移的新型生物标志物和小分子化合物的鉴定
Medicine (Baltimore). 2020 Aug 7;99(32):e21505. doi: 10.1097/MD.0000000000021505.
2
CDK7 inhibitor THZ1 enhances antiPD-1 therapy efficacy via the p38α/MYC/PD-L1 signaling in non-small cell lung cancer.CDK7 抑制剂 THZ1 通过 p38α/MYC/PD-L1 信号通路增强非小细胞肺癌的抗 PD-1 治疗效果。
J Hematol Oncol. 2020 Jul 20;13(1):99. doi: 10.1186/s13045-020-00926-x.
3
DNA methylation-based diagnostic and prognostic biomarkers of nasopharyngeal carcinoma patients.
bioRxiv. 2024 Aug 25:2024.08.24.609537. doi: 10.1101/2024.08.24.609537.
4
Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G/M phase transition regulatory network and the NFκB/JNK signaling pathway.环维黄杨星 D 通过抑制 KIF11-CDC25C-CDK1-CyclinB1 G/M 期转换调控网络和 NFκB/JNK 信号通路抑制非小细胞肺癌细胞的生长和进展。
Int J Oncol. 2023 May;62(5). doi: 10.3892/ijo.2023.5505. Epub 2023 Mar 17.
基于DNA甲基化的鼻咽癌患者诊断和预后生物标志物
Medicine (Baltimore). 2020 Jun 12;99(24):e20682. doi: 10.1097/MD.0000000000020682.
4
High Expression of hsa_circRNA_001387 in Nasopharyngeal Carcinoma and the Effect on Efficacy of Radiotherapy.hsa_circRNA_001387在鼻咽癌中的高表达及其对放射治疗疗效的影响
Onco Targets Ther. 2020 May 8;13:3965-3973. doi: 10.2147/OTT.S249202. eCollection 2020.
5
Circular RNA Expression Profiles in Nasopharyngeal Carcinoma by Sequence Analysis.通过序列分析对鼻咽癌中环状RNA表达谱的研究
Front Oncol. 2020 Apr 30;10:601. doi: 10.3389/fonc.2020.00601. eCollection 2020.
6
SOX9 interacts with FOXC1 to activate MYC and regulate CDK7 inhibitor sensitivity in triple-negative breast cancer.SOX9与FOXC1相互作用，以激活MYC并调节三阴性乳腺癌中CDK7抑制剂的敏感性。
Oncogenesis. 2020 May 12;9(5):47. doi: 10.1038/s41389-020-0232-1.
7
Targeting Super-Enhancers via Nanoparticle-Facilitated BRD4 and CDK7 Inhibitors Synergistically Suppresses Pancreatic Ductal Adenocarcinoma.通过纳米颗粒介导的BRD4和CDK7抑制剂靶向超级增强子协同抑制胰腺导管腺癌
Adv Sci (Weinh). 2020 Feb 16;7(7):1902926. doi: 10.1002/advs.201902926. eCollection 2020 Apr.
8
DNA methylation biomarkers for nasopharyngeal carcinoma.鼻咽癌的 DNA 甲基化生物标志物。
PLoS One. 2020 Apr 9;15(4):e0230524. doi: 10.1371/journal.pone.0230524. eCollection 2020.
9
CDK7 Inhibition is Effective in all the Subtypes of Breast Cancer: Determinants of Response and Synergy with EGFR Inhibition.CDK7 抑制在所有乳腺癌亚型中均有效：对反应的决定因素和与 EGFR 抑制的协同作用。
Cells. 2020 Mar 6;9(3):638. doi: 10.3390/cells9030638.
10
Combination therapy with the CDK7 inhibitor and the tyrosine kinase inhibitor exerts synergistic anticancer effects against MYCN-amplified neuroblastoma.CDK7 抑制剂与酪氨酸激酶抑制剂联合治疗对 MYCN 扩增神经母细胞瘤发挥协同抗癌作用。
Int J Cancer. 2020 Oct 1;147(7):1928-1938. doi: 10.1002/ijc.32936. Epub 2020 Mar 16.