Lu Zhansheng, Liu Bo, Zhang Qinbing, Luo Meng, Sun Yongpan
Department of Thoracic Surgery, Guizhou Provincial People's Hospital, Guiyang 550000, China.
Transl Cancer Res. 2019 Jun;8(3):909-916. doi: 10.21037/tcr.2019.06.01.
The study was designed to investigate the effects of aspirin and hericium polysaccharides (HP) on the expression of CD133 and ABCG2 in lung cancer growth.
, A549 cells were treated with aspirin alone, HP alone, and a combination of both substances. Cell proliferation and the expression of CD133 and ABCG2 were examined using the CCK-8 assay, colony formation assay, and western blot. , eight C57BL/6J male mice were randomly selected as a blank group. The remaining 42 were inoculated with A549 cells to establish tumor-bearing model. The tumor-bearing mice were giving aspirin alone, HP alone, and a combination of both substances and sacrificed after 30 days of drug treatment. After tumor volumes were calculated, and tumor tissues were weighed, Western blot and quantitative fluorescence PCR were used to detect the expression of CD133 and ABCG2 in tumor tissues from different groups.
Aspirin alone, HP alone and combined aspirin and HP inhibited cell proliferation significantly. Western blot results showed that Aspirin alone, HP alone and combined HP and aspirin inhibited the expression levels of CD133 significantly, but HP alone did not inhibit the expression levels of ABCG2. Combined HP and aspirin are synergistic. , HP alone, aspirin alone, and combined aspirin and HP inhibited tumor volume and tumor weights significantly. According to results from the Western blot and PCR analyses, HP alone, aspirin alone, and combined HP and aspirin inhibited the expression levels of CD133 significantly, but HP alone did not inhibit the expression levels of ABCG2. Combined aspirin and HP applications are synergistic.
The combined effect of aspirin and HP inhibited tumor growth significantly by obstructing CD133 and ABCG2 expression in lung cancer.
本研究旨在探讨阿司匹林和猴头菇多糖（HP）对肺癌生长过程中CD133和ABCG2表达的影响。
将A549细胞分别用阿司匹林、HP单独处理以及两种物质联合处理。采用CCK-8法、集落形成试验和蛋白质免疫印迹法检测细胞增殖以及CD133和ABCG2的表达。选取8只C57BL/6J雄性小鼠作为空白组。将其余42只接种A549细胞建立荷瘤模型。给荷瘤小鼠分别单独给予阿司匹林、HP以及两种物质联合使用，药物处理30天后处死。计算肿瘤体积、称量肿瘤组织重量后，采用蛋白质免疫印迹法和定量荧光PCR法检测不同组肿瘤组织中CD133和ABCG2的表达。
单独使用阿司匹林、单独使用HP以及阿司匹林与HP联合使用均显著抑制细胞增殖。蛋白质免疫印迹结果显示，单独使用阿司匹林、单独使用HP以及HP与阿司匹林联合使用均显著抑制CD133的表达水平，但单独使用HP未抑制ABCG2的表达水平。HP与阿司匹林联合使用具有协同作用；单独使用HP、单独使用阿司匹林以及阿司匹林与HP联合使用均显著抑制肿瘤体积和肿瘤重量；根据蛋白质免疫印迹法和PCR分析结果显示，单独使用HP、单独使用阿司匹林以及HP与阿司匹林联合使用均显著抑制CD133的表达水平，但单独使用HP未抑制ABCG2的表达水平；阿司匹林与HP联合使用具有协同作用。
阿司匹林与HP联合作用通过阻碍肺癌中CD133和ABCG2的表达显著抑制肿瘤生长。
