Cheng Yun, Huang Hailiang, Han Yang, Zhu Ying
Department of Gynecology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
Department of Obstetrics & Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Transl Cancer Res. 2020 Nov;9(11):7248-7258. doi: 10.21037/tcr-20-3155.
Yes-associated protein () can function as a cancer suppressor or a cancer promoter. Studies have proved that can collaborate with other genes to accelerate cancerous epithelial to mesenchymal transition (EMT), but little is known about how performs in endometrial carcinoma (EC).
Real time-polymerase chain reaction (RT-PCR) and western blot (WB) were used to quantify the relative mRNA and protein levels of in 50 EC tissue samples and 20 normal endometrial tissues in the proliferative phase. The association between expression level and EC index (clinical stage, histologic grade, and lymphatic metastasis) was analyzed. interference and overexpression vectors were constructed. RT-PCR and WB were used to quantify the mRNA and protein levels of and EMT markers in the normal control group (N), the negative control group (NC), and the interference and overexpression group. Cell counting kit-8 (CCK8) assay and scratch test were performed to evaluate the proliferation, invasion, and migration of EC cells after interference and overexpression.
The mRNA and protein levels of increased in EC tissues (P<0.01) and showed differences associated with histologic grade and lymphatic metastasis, not with the clinical stage (P>0.01). CCK8 assay showed that the proliferation of EC cells was inhibited after interference and increased after overexpression. The cell wound healing test displayed that the migration of EC cells was inhibited after interference and increased after overexpression. RT-PCR and WB found the mRNA and protein levels of E-cadherin (an EMT marker) increased (P<0.01), but those of other markers (N-cadherin, Vimentin) dropped (P<0.01) after interference; however, these trends were inversed after overexpression (P<0.01).
serves as an EC-promoting gene that may regulate the EMT and other EC-related processes via promoting the proliferation, invasion, and migration of EC cells.
Yes相关蛋白（YAP）可作为抑癌基因或癌基因发挥作用。研究表明，YAP可与其他基因协同作用，加速癌上皮细胞向间充质细胞转化（EMT），但YAP在子宫内膜癌（EC）中的作用尚不清楚。
采用实时聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（WB）检测50例EC组织样本和20例增殖期正常子宫内膜组织中YAP的相对mRNA和蛋白水平。分析YAP表达水平与EC指标（临床分期、组织学分级和淋巴转移）之间的关系。构建YAP干扰和过表达载体。采用RT-PCR和WB检测正常对照组（N）、阴性对照组（NC）、YAP干扰组和过表达组中YAP及EMT标志物的mRNA和蛋白水平。采用细胞计数试剂盒-8（CCK8）法和划痕试验评估YAP干扰和过表达后EC细胞的增殖、侵袭和迁移能力。
EC组织中YAP的mRNA和蛋白水平升高（P<0.01），且与组织学分级和淋巴转移相关，与临床分期无关（P>0.01）。CCK8法检测结果显示，YAP干扰后EC细胞增殖受到抑制，过表达后则增强。细胞划痕试验显示，YAP干扰后EC细胞迁移受到抑制，过表达后则增强。RT-PCR和WB检测发现，YAP干扰后E-钙黏蛋白（一种EMT标志物）的mRNA和蛋白水平升高（P<0.01），而其他标志物（N-钙黏蛋白、波形蛋白）的水平下降（P<0.01）；然而，YAP过表达后这些趋势则相反（P<0.01）。
YAP作为一种促进EC的基因，可能通过促进EC细胞的增殖、侵袭和迁移来调节EMT及其他与EC相关的过程。
