Qin Zhaohui, Xie Ruilin, Zhang Wei, Wan Minghui, Zhang Longzheng, Wang Hui, Li Na, Qin Qingwei, Yao Yuanhu
School of Public Health, Xuzhou Medical University, Xuzhou 221000, China.
Department of Radiation Oncology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China.
Transl Cancer Res. 2020 Feb;9(2):639-646. doi: 10.21037/tcr.2019.12.06.
Recent evidences support that low expression of liver kinase B1 (LKB1) triggers epithelial-mesenchymal transition (EMT) through induction of Zinc finger E-box binding homeobox 1 (ZEB1) expression, which downregulates E-cadherin in human lung cancer cell lines. However, the clinicopathological significance of LKB1, EMT, salt-inducible kinase 1 (SIK1), ZEB1 and their relationship in early stage non-small cell lung cancer (ES-NSCLC) patients remain to be determined. In this study, the correlation among expression of LKB1, risk of distant metastasis, prognostic significance, and EMT in ES-NSCLC after surgery was investigated by immunohistochemistry.
Case notes and pathology records of 103 patients with ES-NSCLC were retrospectively analyzed. Immunohistochemical staining was employed to detect LKB1, EMT biomarkers (E-cadherin and vimentin), SIK1 and ZEB1 expression in ES-NSCLC tissues.
LKB1 expression is associated with distant metastasis after treatment (P=0.017). LKB1-high expression group has better overall survival (OS) (P=0.000) and disease-free survival (DFS) (P=0.000). LKB1 expression is correlated with E-cadherin (r=0.231, P=0.019), vimentin (r=-0.225, P=0.022), SIK1 (r=0.218, P=0.027) and ZEB1 (r=-0.242, P=0.014).
Our findings suggest that LKB1-high expression is possibly associated with favourable prognosis and LKB1 expression is correlated with EMT and expression of SIK1 and ZEB1.
近期证据支持，肝激酶B1（LKB1）低表达通过诱导锌指E盒结合同源框1（ZEB1）表达触发上皮-间质转化（EMT），这会下调人肺癌细胞系中的E-钙黏蛋白。然而，LKB1、EMT、盐诱导激酶1（SIK1）、ZEB1在早期非小细胞肺癌（ES-NSCLC）患者中的临床病理意义及其关系仍有待确定。在本研究中，通过免疫组织化学研究了LKB1表达、远处转移风险、预后意义以及EMT在ES-NSCLC术后的相关性。
回顾性分析103例ES-NSCLC患者的病历和病理记录。采用免疫组织化学染色检测ES-NSCLC组织中LKB1、EMT生物标志物（E-钙黏蛋白和波形蛋白）、SIK1和ZEB1的表达。
LKB1表达与治疗后的远处转移相关（P = 0.017）。LKB1高表达组的总生存期（OS）（P = 0.000）和无病生存期（DFS）（P = 0.000）更好。LKB1表达与E-钙黏蛋白（r = 0.231，P = 0.019）、波形蛋白（r = -0.225，P = 0.022）、SIK1（r = 0.218，P = 0.027）和ZEB1（r = -0.242，P = 0.014）相关。
我们的研究结果表明，LKB1高表达可能与良好预后相关，且LKB1表达与EMT以及SIK1和ZEB1的表达相关。
