催乳素（PRL）、胎盘生长因子（PIGF）和神经生长因子受体（NGFR）作为食管鳞状细胞癌（ESCC）患者早期诊断和预后的生物标志物。
Prolactin (PRL), placenta growth factor (PIGF) and nerve growth factor receptor (NGFR) as biomarkers for early diagnosis and prognosis in patients with esophageal squamous cell carcinoma (ESCC).
作者信息
Ai Xiaohong, Zhu Xiaoxi, Zuo Jianhong
机构信息
Department of Radiotherapy, The First Affiliated Hospital, University of South China, Hengyang 421900, China.
Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, School of Medicine, University of South China, Hengyang 421900, China.
出版信息
Transl Cancer Res. 2020 Feb;9(2):647-656. doi: 10.21037/tcr.2019.12.31.
BACKGROUND
The purpose of this study was to investigate the expression and clinical significance of prolactin (PRL), placenta growth factor (PIGF) and nerve growth factor receptor (NGFR) in esophageal squamous cell carcinoma (ESCC).
METHODS
PRL, PIGF and NGFR were selected through being screened normal human and esophageal cancer (EC) plasma by high-throughput protein chips. Subsequently, enzyme linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) were used to detect the expression in ESCC and control group. Then, its clinical significance was statistically evaluated.
RESULTS
The expression of PRL, PIGF and NGFR in plasma and tissue of patients with EC was higher than healthy controls and adjacent tissue, respectively. Among the clinical parameters, the expression of PRL and NGFR protein was correlated with the tumor classification of ESCC (P<0.05), while PIGF protein was correlated with the clinical stage of ESCC (P<0.05). The area under the ROC (AUC) of PRL, PIGF, and NGFR in plasma was 0.69, 0.72, and 0.66 in separately. Furthermore, the combined detection of three proteins had a better AUC of 0.74 with a sensitivity of 66.7% and a specificity of 72.4%. Kaplan-Meier survival analysis revealed that positive expression of PRL, PIGF and NGFR in histological predicted significantly worse overall survival (OS) than negative expression (P<0.05).
CONCLUSIONS
PRL, PIGF and NGFR are promising biomarkers for diagnosis and prognosis prediction of ESCC.
背景
本研究旨在探讨催乳素（PRL）、胎盘生长因子（PIGF）和神经生长因子受体（NGFR）在食管鳞状细胞癌（ESCC）中的表达及其临床意义。
方法
通过高通量蛋白质芯片筛选正常人和食管癌（EC）血浆，选出PRL、PIGF和NGFR。随后，采用酶联免疫吸附测定（ELISA）和免疫组织化学（IHC）检测ESCC组和对照组中的表达情况。然后，对其临床意义进行统计学评估。
结果
EC患者血浆和组织中PRL、PIGF和NGFR的表达分别高于健康对照者和癌旁组织。在临床参数中，PRL和NGFR蛋白的表达与ESCC的肿瘤分级相关（P<0.05），而PIGF蛋白与ESCC的临床分期相关（P<0.05）。血浆中PRL、PIGF和NGFR的ROC曲线下面积（AUC）分别为0.69、0.72和0.66。此外，三种蛋白联合检测的AUC为0.74，敏感性为66.7%，特异性为72.4%。Kaplan-Meier生存分析显示，组织学中PRL、PIGF和NGFR阳性表达者的总生存期（OS）明显低于阴性表达者（P<0.05）。
结论
PRL、PIGF和NGFR是ESCC诊断和预后预测中有前景的生物标志物。
Zotero 插件