Zhao Baolei, Zhu Wentao, Han Xinqiang, Li Xin, Lu Yanmin, Cao Xuefeng, Zhang Fan, Lin Xutao, Zhang Xingyuan, Chen Qiangpu
Department of Hepatobiliary Surgery, Binzhou Medical University Hospital, Binzhou 256603, China.
Department of Gynecology, Binzhou People's Hospital, Binzhou 256603, China.
Transl Cancer Res. 2020 Feb;9(2):1032-1043. doi: 10.21037/tcr.2020.01.02.
(Rupr. & Maxim.) Harms, a traditional Chinese medicine, has been used to treat various diseases, including ischemic, heart diseases, hepatocellular carcinoma (HCC), hypertension and neurasthenia. The purpose of this study was to investigate the anticancer activity of (ASE).
MTT assay, clonogenicity, reverse transcription-polymerase chain reaction (RT-PCR), flow cytometry and Western blot were employed to evaluate the viability and invasion of liver cancer cells. In addition, luciferase assay was used to delineate the inhibitory activity of ethanol extract against NF-κB.
Our results showed that the ethanol extract of ASE could decrease the viability of cancer cells. In addition, the ethanol extract could decrease the protein levels of Matrix metalloproteinase-2 (MMP-2), MMP-9, t-protein kinase B (Akt) and p-Akt, but increase those of E-cadherin. Nuclear factor kappa beta (NF-κB)-Luciferase assay showed the ethanol extract could effectively inhibit the activity of NF-κB. Furthermore, fourteen compounds including seven active compounds were isolated and activities against NF-κB were investigated.
This study confirmed that the ASE could be as an alternative or complementary therapy to treat liver cancer.
（鲁普雷希特和马克西姆）哈姆斯，一种传统中药，已被用于治疗各种疾病，包括缺血性心脏病、肝细胞癌（HCC）、高血压和神经衰弱。本研究的目的是研究（某种物质，原文未明确写出全称，推测为ASE相关物质）的抗癌活性。
采用MTT法、克隆形成实验、逆转录聚合酶链反应（RT-PCR）、流式细胞术和蛋白质印迹法来评估肝癌细胞的活力和侵袭能力。此外，荧光素酶报告基因检测用于描述乙醇提取物对核因子κB（NF-κB）的抑制活性。
我们的结果表明，（某种物质，推测为ASE）的乙醇提取物可降低癌细胞的活力。此外，乙醇提取物可降低基质金属蛋白酶-2（MMP-2）、MMP-9、蛋白激酶B（Akt）和磷酸化Akt的蛋白水平，但增加E-钙黏蛋白的水平。核因子κB（NF-κB）荧光素酶报告基因检测表明乙醇提取物可有效抑制NF-κB的活性。此外，分离出了14种化合物，包括7种活性化合物，并研究了它们对NF-κB的活性。
本研究证实（某种物质，推测为ASE）可作为治疗肝癌的替代或补充疗法。
