Department of Toxicology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China.
J Ethnopharmacol. 2010 Feb 3;127(2):424-32. doi: 10.1016/j.jep.2009.10.022. Epub 2009 Oct 24.
Acanthopanax senticosus (Rupr.et Maxim.) Harms, classified into the family of Araliaceae, is used in a variety of diseases in traditional Chinese system of medicine including hypertension, ischemic heart disease and hepatitis.
Different doses (75 mg/kg, 150 mg/kg and 300 mg/kg) of aqueous extracts of Acanthopanax senticosus Harms were evaluated for the antioxidant activity against oxidative stress in mice induced by tert-butyl hydroperoxide (t-BHP) through observating histopathology of the liver and detecting antioxidant enzyme activity, concentration of antioxidant, and related gene and protein expression.
Acanthopanax senticosus Harms aqueous extracts (ASE) attenuated the morphological injury of liver induced by t-BHP and increased the activity of antioxidant enzymes and the ratio of GSH/GSSG in serum and liver homogenates. Medium and high doses of ASE also elevated the gene expression of NF-E2-related factor-2 (Nrf2), but not CuZnSOD, MnSOD, catalase (CAT), glutathione peroxidase (GPx) and GCLC. Protein expression results showed that Nrf2 and the antioxidant enzymes were all increased significantly by medium and high doses of ASE.
The present results indicated that ASE protect against oxidative stress which may be generated via the induction of Nrf2 and related antioxidant enzymes.
刺五加（Rupr.et Maxim.）Harms，归类为五加科，用于治疗传统中医系统中的多种疾病，包括高血压、缺血性心脏病和肝炎。
通过观察肝脏组织病理学和检测抗氧化酶活性、抗氧化剂浓度以及相关基因和蛋白表达，评估了不同剂量（75mg/kg、150mg/kg 和 300mg/kg）的刺五加 Harms 水提物对叔丁基过氧化物（t-BHP）诱导的小鼠氧化应激的抗氧化活性。
刺五加 Harms 水提物（ASE）减轻了 t-BHP 诱导的肝形态损伤，增加了血清和肝匀浆中抗氧化酶的活性和 GSH/GSSG 的比值。中高剂量的 ASE 还增加了 NF-E2 相关因子-2（Nrf2）的基因表达，但不增加 CuZnSOD、MnSOD、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和 GCLC。蛋白表达结果表明，中高剂量的 ASE 均显著增加了 Nrf2 和抗氧化酶的表达。
本研究结果表明，ASE 可通过诱导 Nrf2 及其相关抗氧化酶来抵抗氧化应激。
