微小RNA-124和微小RNA-378通过上调KiSS1抑制结直肠癌的增殖和侵袭。
MicroRNA-124 and microRNA-378 inhibit the proliferation and invasion of colorectal cancer by upregulating KiSS1.
作者信息
Zheng Yan, Liu Yisu, Lin Yilin, Lin Suyong, Gao Ji, Chen Zhihua, Chen Shaoqin
机构信息
Department of Gastrointestinal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, China.
School of Nursing, Fujian Medical University, Fuzhou 350004, China.
出版信息
Transl Cancer Res. 2020 Apr;9(4):2838-2846. doi: 10.21037/tcr.2020.02.30.
BACKGROUND
The KiSS1 gene is considered a tumor suppressor in various cancers. MicroRNAs are involved in many important life processes, and their regulation of gene expression may be as important as that of transcription factors. Here, we explore the roles of miR-124-3p and miR-378-3p in colorectal cancer and their relationships with the KiSS1 gene.
METHODS
The effects of miR-124-3p and miR-378-3p on KiSS1 protein expression were observed by transfecting colorectal cancer cells (SW-480) with miR-124-3p and miR-378-3p mimics and inhibitors. Moreover, cell proliferation, migration and invasion were evaluated by ethynyl-20-deoxyuridine and Transwell experiments.
RESULTS
The KiSS1 mRNA and protein expression levels were significantly increased in mimic-transfected cells compared with those in untransfected cells, and the proliferation, migration and invasion abilities of the former were decreased; in addition, opposing results were obtained in the inhibitor and mimic groups.
CONCLUSIONS
In conclusion, our studies indicate that miR-124-3p and miR-378-3p upregulate the expression of KiSS1 and are associated with colorectal cancer metastasis and progression. miR-124-3p, miR-378-3p and KiSS1 may play important roles in colorectal cancer.
背景
KiSS1基因在多种癌症中被认为是一种肿瘤抑制基因。微小RNA参与许多重要的生命过程，它们对基因表达的调控可能与转录因子一样重要。在此，我们探讨miR-124-3p和miR-378-3p在结直肠癌中的作用及其与KiSS1基因的关系。
方法
通过用miR-124-3p和miR-378-3p模拟物和抑制剂转染结直肠癌细胞（SW-480），观察miR-124-3p和miR-378-3p对KiSS1蛋白表达的影响。此外，通过乙炔基-20-脱氧尿苷和Transwell实验评估细胞增殖、迁移和侵袭能力。
结果
与未转染细胞相比，模拟物转染细胞中KiSS1 mRNA和蛋白表达水平显著升高，且前者的增殖、迁移和侵袭能力降低；此外，抑制剂组和模拟物组得到了相反的结果。
结论
总之，我们的研究表明，miR-124-3p和miR-378-3p上调KiSS1的表达，并与结直肠癌转移和进展相关。miR-124-3p、miR-378-3p和KiSS1可能在结直肠癌中发挥重要作用。
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