da Silva Giselle Diniz Guimarães, Pinheiro Tiago Novaes
Public Health Leônidas and Maria Deane-Fiocruz Amazônia, Manaus-Am, Brazil.
Surgical Pathology and Oral and Maxillofacial Pathology Service at Amazonas State University, Manaus-Am, Brazil.
Transl Cancer Res. 2020 Apr;9(4):3101-3106. doi: 10.21037/tcr.2019.12.23.
Oral leukoplakia is the most common potentially malignant lesion, the etiology of this change is still uncertain which makes it difficult to predict when this will undergo malignant transformation. Recent studies have examined the involvement of mast cells in this process, it is believed that these cells through the release of pro-angiogenic factors influence the processing mechanism of such lesions through promoting angiogenesis. It was the objective of this study to evaluate the inflammatory process in oral leukoplakia comparing it to a non-cancerous lesion correlating the intensity of mast cells with inflammatory infiltrate and vascularization in these lesions.
A total of 20 selected cases divided into two groups, oral leukoplakia and inflammatory fibrous hyperplasia (IFH). Histochemical Technique of Toluidine Blue was applied to enhance mast cells and histomorphometry for quantifying mast cells, blood vessels and inflammatory cell infiltration by capturing digital images of the samples and analysis software Image Java version 1.48v. Mast cells and blood vessels were counted in 10 different areas and the intensity of the inflammatory infiltrate was measured in three areas. The analysis of variance (ANOVA) was used for between-group variation, a P value of 0.05 for statistical significance.
It was observed a reduction in the concentration of mast cells in oral leukoplakia compared fibrous inflammatory hyperplasia. In contrast to the concentration of blood vessels were present in higher concentrations in the Oral leukoplakia lesions. The inflammatory infiltrate in most cases was considered scarce, except in a case where that IFH was intense and a case of moderate inflammatory infiltrate in oral leukoplakia.
The results showed reduction in the number of mast cells in the oral leukoplakia and greater intensity in the inflammatory process in the IFH, suggesting a possible carcinogenic influence in the microenvironment oral leukoplakia.
口腔白斑是最常见的潜在恶性病变，这种病变的病因仍不确定，这使得难以预测其何时会发生恶变。最近的研究探讨了肥大细胞在这一过程中的作用，据信这些细胞通过释放促血管生成因子，促进血管生成，从而影响此类病变的发展机制。本研究的目的是评估口腔白斑中的炎症过程，并将其与非癌性病变进行比较，将肥大细胞的数量与这些病变中的炎症浸润及血管形成强度相关联。
总共选取20例病例分为两组，即口腔白斑组和炎性纤维性增生（IFH）组。应用甲苯胺蓝组织化学技术来增强肥大细胞显示，并采用组织形态计量学方法，通过采集样本的数字图像并使用Image Java 1.48v分析软件，对肥大细胞、血管和炎性细胞浸润进行定量分析。在10个不同区域计数肥大细胞和血管，并在3个区域测量炎症浸润的强度。采用方差分析（ANOVA）分析组间差异，P值小于0.05具有统计学意义。
观察到与炎性纤维性增生相比，口腔白斑中肥大细胞的浓度降低。相反，口腔白斑病变中血管的浓度较高。在大多数病例中，炎症浸润被认为较少，除了1例IFH严重的病例和1例口腔白斑中度炎症浸润的病例。
结果显示口腔白斑中肥大细胞数量减少，而IFH中的炎症过程强度更大，提示口腔白斑微环境中可能存在致癌影响。
