Oral Pathology, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
Arch Oral Biol. 2011 Mar;56(3):231-7. doi: 10.1016/j.archoralbio.2010.09.020. Epub 2010 Oct 29.
This study analysed the immunohistochemical expression of mast cell tryptase in giant cell fibromas (GCFs). In addition, the possible interaction of mast cells with stellate giant cells, as well as their role in fibrosis and tumour progression, was investigated. For this purpose, the results were compared with cases of inflammatory fibrous hyperplasia (IFH) and normal oral mucosa. Thirty cases of GCF, 30 cases of IFH and 10 normal mucosa specimens used as control were selected. Immunoreactivity of mast cells to the anti-tryptase antibody was analysed quantitatively in the lining epithelium and in connective tissue. In the epithelial component (p=0.250) and connective tissue (p=0.001), the largest mean number of mast cells was observed in IFHs and the smallest mean number in GCFs. In connective tissue, the mean percentage of degranulated mast cells was higher in GCFs than in IFHs and normal mucosa specimens (p<0.001). Analysis of the percentage of degranulated mast cells in areas of fibrosis and at the periphery of blood vessels also showed a larger mean number in GCFs compared to IFHs and normal mucosa specimens (p<0.001). The percent interaction between mast cells and stellate giant cells in GCFs was 59.62%. In conclusion, although mast cells were less numerous in GCFs, the cells exhibited a significant interaction with stellate giant cells present in these tumours. In addition, the results suggest the involvement of mast cells in the induction of fibrosis and modulation of endothelial cell function in GCFs.
本研究分析了组织肥大细胞 tryptase 在巨大细胞纤维瘤（GCFs）中的免疫组织化学表达。此外，还研究了肥大细胞与星状巨细胞的可能相互作用，以及它们在纤维化和肿瘤进展中的作用。为此，将结果与炎性纤维增生（IFH）和正常口腔黏膜的病例进行了比较。选择了 30 例 GCF、30 例 IFH 和 10 例正常口腔黏膜作为对照。用抗 tryptase 抗体对肥大细胞的免疫反应进行了定量分析，分析了衬里上皮和结缔组织中的肥大细胞。在上皮成分（p=0.250）和结缔组织（p=0.001）中，IFH 中观察到的肥大细胞数量最多，而 GCF 中观察到的肥大细胞数量最少。在结缔组织中，与 IFH 和正常黏膜标本相比，GCF 中脱颗粒肥大细胞的平均百分比更高（p<0.001）。对纤维化区域和血管周围脱颗粒肥大细胞百分比的分析也显示，与 IFH 和正常黏膜标本相比，GCF 中的平均数量更多（p<0.001）。GCF 中肥大细胞与星状巨细胞的相互作用百分比为 59.62%。结论：尽管 GCF 中的肥大细胞数量较少，但这些细胞与存在于这些肿瘤中的星状巨细胞之间存在显著的相互作用。此外，研究结果表明肥大细胞参与了 GCF 中纤维化的诱导和内皮细胞功能的调节。
