小型猪模型中灭活自体皮下脂肪组织和皮肤真皮组织制备假体的实验研究
An experimental study on the prosthesis produced by inactivated autologous subcutaneous adipose tissue and skin dermal tissue in a mini-pig model.
作者信息
Jiang Chuner, Pan Yongming, Huang Junjie, He Xiangming, Ni Jianfen, Ding Xiaowen, Zou Dehong, Wang Fangzheng
机构信息
Department of Breast Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Hangzhou, China.
Department of Breast Surgery, Zhejiang Cancer Hospital, Hangzhou, China.
出版信息
Transl Cancer Res. 2020 Aug;9(8):4770-4780. doi: 10.21037/tcr-19-2844.
BACKGROUND
Autologous adipose tissue grafting is a valuable strategy for breast reconstruction in breast cancer patients. However, adipose tissue is absorbed and liquefies easily, and the cosmetic effects do not last. The purpose of this study is to evaluate the cosmetic effects and histopathologic changes of the prostheses produced by inactivated autologous subcutaneous adipose tissue and dermal outer capsule in the mini-pig model.
METHODS
This prospective study was conducted in 6 Bama adult female mini-pigs weighing 15.5-20 kg. Autologous subcutaneous adipose tissue and dermis were harvested and made into the different prostheses. Then, the prostheses were implanted into abdominal subcutaneous tissue of the mini-pigs. At the 14 and 30th day after implantation, the skin appearances were observed, and the prostheses were harvested. The histopathologic changes of adipocytes and dermis in the different prostheses were evaluated.
RESULTS
experiments showed that there were no significant changes in the size and histomorphology of autologous adipose tissue and/or dermis in the different prostheses. However, the results of the experiment found that the prosthesis produced by the inactivated adipose tissue with a dermal outer capsule produced the best cosmetic effect and the least fibrous connective tissue proliferation around the prosthesis. Moreover, the fewest proliferative fibrous connective tissues and infiltrated inflammatory cells were found in the adipocytes wrapped by dermis. The adipocyte morphology was normal.
CONCLUSIONS
Results in this animal model indicated that the prosthesis produced by inactivated autologous subcutaneous adipose tissue with a dermal outer capsule may be a promising prosthesis for breast reconstruction. Validation of this new prosthesis requires more experiments to assess the long-term cosmetic effects and histopathologic changes.
背景
自体脂肪组织移植是乳腺癌患者乳房重建的一种有价值的策略。然而，脂肪组织容易被吸收和液化，美容效果不能持久。本研究的目的是在小型猪模型中评估由灭活的自体皮下脂肪组织和真皮外膜制成的假体的美容效果和组织病理学变化。
方法
本前瞻性研究在6只体重15.5 - 20千克的巴马成年雌性小型猪中进行。采集自体皮下脂肪组织和真皮并制成不同的假体。然后，将假体植入小型猪的腹部皮下组织。在植入后的第14天和第30天，观察皮肤外观并取出假体。评估不同假体中脂肪细胞和真皮的组织病理学变化。
结果
实验表明，不同假体中自体脂肪组织和/或真皮的大小和组织形态没有显著变化。然而，实验结果发现，带有真皮外膜的灭活脂肪组织制成的假体产生了最佳的美容效果，假体周围的纤维结缔组织增殖最少。此外，在被真皮包裹的脂肪细胞中发现的增殖性纤维结缔组织和浸润的炎症细胞最少。脂肪细胞形态正常。
结论
该动物模型的结果表明，由灭活的自体皮下脂肪组织和真皮外膜制成的假体可能是一种有前景的乳房重建假体。这种新假体的验证需要更多实验来评估长期美容效果和组织病理学变化。
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