Koch G L, Smith M J
Br J Cancer. 1986 Jan;53(1):13-22. doi: 10.1038/bjc.1986.3.
Con A acceptor glycoproteins from the human Molt 4 (T cell leukaemia) and HeLa (endocervical adenocarcinoma) cell lines were purified by affinity chromatography and used for the preparation of rat antisera. Cross-absorption analysis showed that each antiserum contained antibodies which recognised cell surface antigens preferentially expressed by the donor cell line. Molt 4-associated antigens were fully expressed on T cell tumour lines and normal thymocytes, but not on non T cell tumour lines, peripheral blood lymphocytes or other blood cells. Immunofluorescence studies showed that the antigens were preferentially expressed on a sub-population of immature thymocytes. HeLa-associated antigens were only fully expressed on one other epithelial tumour cell in a panel of 17 cell lines. Immunofluorescence studies showed that the HeLa-associated antigens were expressed on normal endocervical adenoepithelium but not on ectocervical, endometrial or intestinal epithelia. Thus purified Con A acceptor glycoproteins of cultured tumour cell lines are potent immunogens for the generation of antibodies recognising lineage-associated differentiation antigens. These antigens should be useful in tumour classification and in the study of normal differentiation.
通过亲和层析法纯化了来自人Molt 4（T细胞白血病）和HeLa（宫颈腺癌）细胞系的刀豆蛋白A受体糖蛋白，并用于制备大鼠抗血清。交叉吸收分析表明，每种抗血清都含有能识别供体细胞系优先表达的细胞表面抗原的抗体。与Molt 4相关的抗原在T细胞肿瘤系和正常胸腺细胞上完全表达，但在非T细胞肿瘤系、外周血淋巴细胞或其他血细胞上不表达。免疫荧光研究表明，这些抗原优先在未成熟胸腺细胞的一个亚群上表达。在一组17种细胞系中，与HeLa相关的抗原仅在另一种上皮肿瘤细胞上完全表达。免疫荧光研究表明，与HeLa相关的抗原在正常宫颈腺上皮上表达，但在外宫颈、子宫内膜或肠上皮上不表达。因此，培养的肿瘤细胞系纯化的刀豆蛋白A受体糖蛋白是产生识别谱系相关分化抗原的抗体的有效免疫原。这些抗原在肿瘤分类和正常分化研究中应会有用。
