Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
Department of Clinical Medicine, Changde Vocational and Technical College, Changde, Hunan 415000, China.
Aging (Albany NY). 2022 Feb 4;14(3):1374-1388. doi: 10.18632/aging.203879.
The clinical prognosis of breast cancer is closely related to its infiltrating immune status. The study sought to explore tumor-infiltrating immune cells (TILs) and immune-associated genes in the tumor microenvironment of breast invasive carcinoma (BRCA). The ESTIMATE algorithm was used to evaluate the microenvironment of breast cancer patients in TCGA database. The tumor's matrix score and immune score were obtained. The median was divided into two sub groups according to the median of the score, and the correlation between the score and prognosis was also discussed. Differentially expressed genes were screened from two subgroups with high and low score of breast cancer, and the differentially expressed genes were analyzed by GO and KEGG enrichment to explore their possible molecular functions, biological processes, cellular components and signal pathways involved in gene enrichment. It was found that there was a significant correlation between immune score and five-year survival rate, and the high score group had a better prognosis. Macrophage M1 and T cell CD8+ cells were positively related to 5-year overall survival in patients with breast invasive carcinoma. However, Macrophage M2 was negatively related to 5-year overall survival. We also observed that the low expression of four genes (CLEC3A, MCTS1, PDP1 and TCP1,) was related to favorable survival outcomes. High expression of FOXP3, CXCL9, CCR5, CXCR3, and CD37 was related to a high overall survival rate in BRCA. We identified a list of immune - related cells and genes that are useful for Prognostic evaluation and individualized treatment of BRCA.
乳腺癌的临床预后与浸润性免疫状态密切相关。本研究旨在探讨乳腺浸润性癌（BRCA）肿瘤微环境中的肿瘤浸润免疫细胞（TILs）和免疫相关基因。采用 ESTIMATE 算法评估 TCGA 数据库中乳腺癌患者的微环境，获得肿瘤基质评分和免疫评分。根据评分中位数将肿瘤分为高低两组亚组，并讨论评分与预后的相关性。从乳腺癌高低评分的两组中筛选差异表达基因，对差异表达基因进行 GO 和 KEGG 富集分析，探讨其可能的分子功能、生物学过程、细胞组成和信号通路。结果发现，免疫评分与 5 年生存率显著相关，高评分组预后较好。巨噬细胞 M1 和 T 细胞 CD8+细胞与乳腺浸润性癌患者的 5 年总生存率呈正相关，而巨噬细胞 M2 与 5 年总生存率呈负相关。我们还观察到，四种基因（CLEC3A、MCTS1、PDP1 和 TCP1）的低表达与良好的生存结局相关。FOXP3、CXCL9、CCR5、CXCR3 和 CD37 的高表达与 BRCA 的高总生存率相关。我们确定了一组与预后评估和 BRCA 个体化治疗相关的免疫相关细胞和基因。
