Campistron G, Buijs R M, Geffard M
Brain Res. 1986 Jun 25;376(2):400-5. doi: 10.1016/0006-8993(86)90208-8.
Antibodies were raised against glycine and they were specific for immunocytochemistry. Obtained from rabbits immunized with glycine conjugated to glutaryled protein-carriers, antisera were then purified by adsorption on the various glutaraldehyde-conjugated protein-carriers. Using a modified ELISA method, their specificity was determined in competition experiments between conjugated glycine and either non-conjugated glycine or other conjugated amino acids or derivatives, preincubated with anti-glycine antibodies. Calculated at half-displacement, the resulting cross-reactivity ratios showed conjugated glycine to be the best recognized compound. By revealing the presence of the majority of the glycine-containing cell bodies in the brainstem and spinal cord, immunocytochemical applications of glycine antibodies confirmed their use as specific tools for a better understanding of the role of glycine in the central nervous system.
制备了针对甘氨酸的抗体，这些抗体对免疫细胞化学具有特异性。抗血清取自用与戊二酰化蛋白质载体偶联的甘氨酸免疫的兔子，然后通过吸附在各种戊二醛偶联的蛋白质载体上进行纯化。使用改良的ELISA方法，在与抗甘氨酸抗体预孵育的偶联甘氨酸与未偶联甘氨酸或其他偶联氨基酸或衍生物之间的竞争实验中确定其特异性。以半置换计算，所得的交叉反应率表明偶联甘氨酸是最易被识别的化合物。通过揭示脑干和脊髓中大多数含甘氨酸细胞体的存在，甘氨酸抗体的免疫细胞化学应用证实了它们作为更好理解甘氨酸在中枢神经系统中作用的特异性工具的用途。
