Prof. Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Kallam Anji Reddy Campus, Hyderabad, Telangana, 500034, India.
Srimati Kanuri Santhamma Centre for Vitreo Retinal Diseases, L. V. Prasad Eye Institute, Kallam Anji Reddy Campus, Hyderabad, Telangana, 500034, India.
Exp Eye Res. 2022 Apr;217:108968. doi: 10.1016/j.exer.2022.108968. Epub 2022 Feb 1.
The focus of the current review is multi-fold and compares the diversity and abundance of fungi on the ocular surface by the conventional culture-based method with the more sensitive, high throughput, culture-independent NGS method. The aim is to highlight the existence of a core ocular mycobiome and explore the transition of the ocular fungal microbiota from the normal eye to the diseased eye. PubMed, Google Scholar and Medline were used to search for publications and reviews related to cultivable fungi and the mycobiome of the normal and diseased eye. The conventional cultivable approach and the NGS approach confirm that the eye has its own mycobiome and several confounding factors (age, gender, ethnicity etc.) influence the mycobiome. Further, dysbiosis in the mycobiome appears to be associated with ocular diseases and thus impacts the health of the human eye. Considering that the mycobiome of the eye is influenced by several confounding factors and also varies with respect to the disease status of the eye there is a need to extensively explore the mycobiome under different physiological conditions, different ethnicities, geographical regions etc. Such studies would unravel the diversity and abundance of the mycobiomes and contribute to our understanding of ocular health. Research focused on ocular mycobiomes may eventually help to build a targeted and individualized treatment.
本综述的重点是多方面的，通过传统的基于培养的方法与更敏感、高通量、不依赖培养的 NGS 方法比较了眼部表面真菌的多样性和丰度。目的是强调眼表核心真菌组的存在，并探索眼部真菌菌群从正常眼向患病眼的转变。本文通过 PubMed、Google Scholar 和 Medline 检索了与可培养真菌和正常及患病眼的真菌组相关的研究论文和综述。传统的可培养方法和 NGS 方法均证实，眼睛具有其自身的真菌组，而几个混杂因素（年龄、性别、种族等）影响真菌组。此外，真菌组的失调似乎与眼部疾病有关，从而影响人类眼睛的健康。鉴于眼部真菌组受多种混杂因素的影响，并且还随眼部疾病状态的变化而变化，因此需要在不同的生理条件、不同的种族、不同的地理区域等条件下广泛探索真菌组。这些研究将揭示真菌组的多样性和丰度，并有助于我们了解眼部健康。专注于眼部真菌组的研究最终可能有助于建立有针对性和个体化的治疗方法。
