School of Food Safety, Taipei Medical University, Taipei 11031, Taiwan.
Graduate Institute of Food Science and Technology, National Taiwan University, 1 Roosevelt Rd., Sec. 4, Taipei 10617, Taiwan.
Int J Biol Macromol. 2022 Apr 15;204:136-143. doi: 10.1016/j.ijbiomac.2022.01.186. Epub 2022 Feb 1.
Cellulosic waste as a major type of agricultural waste can be acid deconstructed as a carbon source for fermentation application. However, various fermented inhibitors, such as formic acid, furfural, and 5-hydroxymethylfurfural, are also produced during processing. In this study, sugarcane bagasse (SB) was hydrolyzed with sulfuric acid, and atmospheric cold plasma (ACP) was used to remove the toxic inhibitors. The detoxified SB hydrolysate was used as alternative nutrients for bacterial cellulose (BC) production. Results showed that degradation rates of formic acid, 5-hydroxymethylfurfural, and furfural respectively reached 25.2%, 78.6%, and 100% with optimized ACP conditions (argon ACP at 200 W for 25 min). In BC production, the ACP-treated SB hydrolysate group (PT) exhibited high BC production (1.68 g/L) but was lower than that from the ACP-untreated SB hydrolysate group (PUT) (1.88 g/L), which suggests that ACP detoxification might also cause some crucial nutrients loss of the SB hydrolysate, leading to a decrease in BC production. The material properties of BC produced from detoxified based medium are also evaluated. These findings have important implications for the broader domain of ACP detoxification for cellulosic acid hydrolysates applied to BC production.
纤维素废物作为主要的农业废物类型，可以被酸解作为发酵应用的碳源。然而，在加工过程中也会产生各种发酵抑制剂，如甲酸、糠醛和 5-羟甲基糠醛。在本研究中，使用硫酸水解甘蔗渣（SB），并采用常压冷等离子体（ACP）去除有毒抑制剂。经解毒的 SB 水解物可作为生产细菌纤维素（BC）的替代营养物。结果表明，在优化的 ACP 条件下（氩气 ACP 在 200 W 下处理 25 分钟），甲酸、5-羟甲基糠醛和糠醛的降解率分别达到 25.2%、78.6%和 100%。在 BC 生产中，经 ACP 处理的 SB 水解物组（PT）表现出较高的 BC 产量（1.68 g/L），但低于未经 ACP 处理的 SB 水解物组（PUT）（1.88 g/L），这表明 ACP 解毒可能也会导致 SB 水解物中一些关键营养物质的损失，从而降低 BC 的产量。还评估了基于解毒的培养基生产的 BC 的材料性能。这些发现对更广泛的纤维素酸水解物的 ACP 解毒应用于 BC 生产领域具有重要意义。
