Zvaigzne G V, Brogan D A, Bernstein L H
Clin Chem. 1986 Mar;32(3):437-40.
Derivation of standard curves for the EMIT therapeutic drug monitoring system involves several mathematical algorithms, all of which can be rewritten in the form of a linear equation y = mx + b. We examined the stability of the standard curve in terms of slope and y-intercept for three drug assays (procainamide, gentamicin, and carbamazepine) by generating calibration curves intermittently for periods as long as 90 days. Controls at three concentrations were assayed after each calibration to validate the standard curves. On the basis of 98% confidence intervals, the slopes of standard curves for procainamide, gentamicin, and carbamazepine were stable for 89, 80, and 57 days, respectively. Control values generated from standard-curve manipulations (adjustments to the y-intercept) indicated consistent accuracy and precision throughout the entire study, as compared with control values determined after each calibration. The increased utility of the standard curve and reagents suggests that full recalibration on a regular basis is not always necessary.
EMIT治疗药物监测系统标准曲线的推导涉及多种数学算法，所有这些算法都可以改写为线性方程y = mx + b的形式。我们通过间歇性生成长达90天的校准曲线，研究了三种药物检测（普鲁卡因胺、庆大霉素和卡马西平）标准曲线在斜率和y轴截距方面的稳定性。每次校准后对三种浓度的对照品进行检测，以验证标准曲线。基于98%置信区间，普鲁卡因胺、庆大霉素和卡马西平标准曲线的斜率分别在89天、80天和57天内保持稳定。与每次校准后确定的对照品值相比，通过标准曲线操作（调整y轴截距）生成的对照品值在整个研究过程中显示出一致的准确性和精密度。标准曲线和试剂效用的提高表明，不一定总是需要定期进行全面重新校准。
