Iosefsohn M, Boeckx R L, Hicks J M
Ther Drug Monit. 1981;3(4):365-70. doi: 10.1097/00007691-198104000-00007.
We have adapted the Syva Emit gentamicin and tobramycin procedure to the IL microcentrifugal analyzer, MCA III. With this instrument the recommended instructions for reagent preparation and reaction sequence did not produce a useable standard curve. We modified the dilutions and volumes for the working reagents and reversed the sequence of addition of the working reagents. WE assessed the linearity and precision of the analysis and compared the Syva gentamicin and tobramycin Emit procedure on the IL MCA III with the semiautomated procedure on a Gilford Stasar III spectrophotometer. With the IL MCA III only 3 to 5 microliter of serum is used, 17 analyses can be obtained in 8 min, and the reagent costs are reduced considerably.
我们已将Syva Emit庆大霉素和妥布霉素检测方法适配于IL微量离心分析仪MCA III。使用该仪器时，按照推荐的试剂制备说明和反应顺序无法生成可用的标准曲线。我们修改了工作试剂的稀释度和体积，并颠倒了工作试剂的添加顺序。我们评估了该分析方法的线性和精密度，并将IL MCA III上的Syva庆大霉素和妥布霉素Emit检测方法与吉尔福德Stasar III分光光度计上的半自动检测方法进行了比较。使用IL MCA III时，仅需3至5微升血清，8分钟内可进行17次分析，且试剂成本大幅降低。
