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从发酵茶叶中分离出的潜在益生菌CRM56-2的基因组评估

Genomic Assessment of Potential Probiotic CRM56-2 Isolated from Fermented Tea Leaves.

作者信息

Kingkaew Engkarat, Tanaka Naoto, Shiwa Yuh, Sitdhipol Jaruwan, Nuhwa Rattanatda, Tanasupawat Somboon

机构信息

Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

Department of Biology, School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.

出版信息

Trop Life Sci Res. 2024 Jul;35(2):249-269. doi: 10.21315/tlsr2024.35.2.12. Epub 2024 Jul 31.

DOI:10.21315/tlsr2024.35.2.12
PMID:39234476
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11371405/
Abstract

is a widely studied species known for its probiotic properties that can help alleviate serum cholesterol levels. Whole-genome sequencing provides genetic information on probiotic attributes, metabolic activities and safety assessment. This study investigates the probiotic properties of strain CRM56-2, isolated from Thai fermented tea leaves, using Whole-Genome Sequencing (WGS) to evaluate the safety, health-promoting genes and functional analysis. Strain CRM56-2 showed bile salt hydrolase (BSH) activity, assimilated cholesterol at a rate of 75.94%, tolerated acidic and bile environments and attached to Caco-2 cells. Based on ANIb (98.9%), ANIm (99.2%), and digital DNA-DNA hybridisation (98.3%), strain CRM56-2 was identified as . analysis revealed that it was not pathogenic and contained no antibiotic-resistance genes or plasmids. CRM56-2 possessed genes linked to several probiotic properties and beneficial impacts. The genome of strain CRM56-2 suggested that CRM56-2 is non-hazardous, with potential probiotic characteristics and beneficial impacts, which could enhance its probiotic application. Consequently, CRM56-2 demonstrated excellent cholesterol-lowering activity and probiotic properties.

摘要

是一种因具有益生菌特性而被广泛研究的物种，这些特性有助于降低血清胆固醇水平。全基因组测序提供了有关益生菌属性、代谢活动和安全性评估的遗传信息。本研究使用全基因组测序（WGS）来评估从泰国发酵茶叶中分离出的菌株CRM56-2的益生菌特性，以进行安全性、促进健康基因及功能分析。菌株CRM56-2显示出胆汁盐水解酶（BSH）活性，以75.94%的速率同化胆固醇，耐受酸性和胆汁环境，并附着于Caco-2细胞。基于ANIb（98.9%）、ANIm（99.2%）和数字DNA-DNA杂交（98.3%），菌株CRM56-2被鉴定为 。分析表明它无致病性，且不含有抗生素抗性基因或质粒。CRM56-2拥有与多种益生菌特性和有益影响相关的基因。菌株CRM56-2的基因组表明CRM56-2无危害，具有潜在的益生菌特性和有益影响，这可能会增强其益生菌应用。因此，CRM56-2表现出优异的降胆固醇活性和益生菌特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d233/11371405/4dd01416c242/TLSR-35-2-249-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d233/11371405/1c854d5d6e5e/TLSR-35-2-249-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d233/11371405/4dd01416c242/TLSR-35-2-249-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d233/11371405/1c854d5d6e5e/TLSR-35-2-249-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d233/11371405/4dd01416c242/TLSR-35-2-249-g002.jpg

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