Laboratorio de Acarología, Departamento de Biología Comparada, Facultad de Ciencias, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, 04510 Ciudad de México, Mexico.
Laboratorio de Acarología, Departamento de Biología Comparada, Facultad de Ciencias, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, 04510 Ciudad de México, Mexico.
Ticks Tick Borne Dis. 2022 May;13(3):101911. doi: 10.1016/j.ttbdis.2022.101911. Epub 2022 Jan 31.
The present study analyzes the diversity of bacteria of the genus Rickettsia in ticks collected from wild mammals and vegetation at the Chamela Biological Station (EBCh), Jalisco, Mexico, a core area in the Chamela-Cuixmala Biosphere Reserve, which is characterized by the presence of tropical dry forest, one of the most threatened ecosystems in the world. Ticks were collected during April and November 2018 and 2019, determined to species, and subsequently processed for rickettsiae. Genomic DNA was extracted from each of 349 ticks (198 from mammals, 151 from vegetation) and screened for Rickettsia by targeting the gltA and ompA genes. Overall, 75 ticks (21.5%) were positive for rickettsiae (46 from mammals, 29 from vegetation). Through BLASTn and maximum likelihood analysis, four Rickettsia taxa were identified. Only Rickettsia amblyommatis had been previously recorded in the vicinity of the EBCh, and we confirm its presence in this reserve, associated with Amblyomma mixtum, Amblyomma cf. oblongoguttatum and Amblyomma parvum. Additionally, we record for the first time at EBCh the taxa Rickettsia parkeri strain Atlantic rainforest, which is known to cause spotted fever illness in humans, detected in Amblyomma ovale; Rickettsia bellii in A. parvum and Ornithodoros talaje; and an undescribed Rickettsia sp. in Ixodes sinaloa.
本研究分析了从墨西哥哈利斯科州查米拉生物站（EBCh）的野生哺乳动物和植被中采集的蜱虫携带的立克次体属细菌的多样性。查米拉-奎萨马拉生物圈保护区是一个核心区域，以热带干旱森林为特征，这是世界上最受威胁的生态系统之一。蜱虫于 2018 年 4 月和 2019 年 11 月采集，并按物种确定，随后用于检测立克次体。从 349 只蜱虫（198 只来自哺乳动物，151 只来自植被）中提取基因组 DNA，并针对 gltA 和 ompA 基因筛选立克次体。总体而言，75 只蜱虫（21.5%）检测到立克次体阳性（46 只来自哺乳动物，29 只来自植被）。通过 BLASTn 和最大似然分析，鉴定了 4 种立克次体分类群。仅以前在 EBCh 附近记录过的立克次体属 Amblyommatis，我们确认其在该保护区存在，与 Amblyomma mixtum、 Amblyomma cf. oblongoguttatum 和 Amblyomma parvum 相关。此外，我们首次在 EBCh 记录了立克次体属帕克氏菌种大西洋雨林，已知其可引起人类斑疹热疾病，在 Amblyomma ovale 中检测到；在 A. parvum 和 Ornithodoros talaje 中检测到 Rickettsia bellii；在 Ixodes sinaloa 中检测到一种未描述的 Rickettsia sp.。
