Peña-Bates Carlos A, Lugo-Caballero Cesar I, Pavía-Ruz Norma, Omodior Oghenekaro, Noh-Pech Henry R, Puerto-Manzano Fernando I, Dzul-Rosado Karla R
Centro de Investigaciones Regionales "Dr. Hideyo Noguchi," Universidad Autónoma de Yucatán, Mérida, México.
Health Affairs Institute, West Virginia University, Charleston, West Virginia.
Am J Trop Med Hyg. 2025 Jan 28;112(4):909-915. doi: 10.4269/ajtmh.24-0491. Print 2025 Apr 2.
The socioecological conditions of Mexican regions are conducive to the spread of vector-borne diseases. Although there are established treatment guidelines for dengue and rickettsiosis, diagnosis is complicated. The objective of this work was to identify epitopes of Rickettsia and dengue virus that could be used in serology screening against vector-borne diseases. For this, epitopes with high histocompatibility complex class II binding efficiency of OmpB protein of Rickettsia rickettsii and NS2B protein of dengue virus were identified in silico through a reverse vaccinology strategy. The selected epitopes were grouped into multipeptide sequences that were synthesized and immobilized in a nitrocellulose membrane to evaluate the reactivity sera from patients previously infected with dengue or Rickettsia. The evaluation of the sequences of the NS2B and OmpB proteins was performed with 60 sera previously diagnosed as positive or negative by the respective gold standard techniques. The dot blot technique was used for the antigenic evaluation of the peptides against these serum samples. Dot blot analysis correctly identified 85% of sera positive for rickettsiosis and 75% of sera positive for dengue. Experimental evidence from multipeptide sequences suggests their potential use in the development of diagnostic tests for dengue and rickettsiosis.
墨西哥各地区的社会生态条件有利于媒介传播疾病的传播。尽管已有登革热和立克次体病的既定治疗指南，但诊断仍很复杂。这项工作的目的是确定立克次体和登革热病毒的表位，可用于针对媒介传播疾病的血清学筛查。为此，通过反向疫苗学策略在计算机上鉴定了立氏立克次体的OmpB蛋白和登革热病毒的NS2B蛋白具有高二类组织相容性复合体结合效率的表位。将选定的表位分组为多聚肽序列，合成并固定在硝酸纤维素膜上，以评估先前感染登革热或立克次体的患者血清的反应性。使用60份先前通过各自的金标准技术诊断为阳性或阴性的血清对NS2B和OmpB蛋白的序列进行评估。斑点印迹技术用于针对这些血清样本对肽进行抗原性评估。斑点印迹分析正确鉴定出85%的立克次体病阳性血清和75%的登革热阳性血清。来自多聚肽序列的实验证据表明它们在登革热和立克次体病诊断测试开发中的潜在用途。
