巴基斯坦卡拉奇市商业蛋鸡中H5、H7和H9型禽流感病毒的流行情况。
Prevalence of avian influenza H5, H7, and H9 viruses in commercial layers in Karachi, Pakistan.
作者信息
Channa A A, Tariq M, Nizamani Z A, Kalhoro N H
机构信息
Department of Veterinary Pathology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan.
Research and Development Section, Sindh Institute of Animal Health, Karachi, Pakistan.
出版信息
Iran J Vet Res. 2021 Fall;22(4):352-355. doi: 10.22099/IJVR.2021.41104.5964.
BACKGROUND
Avian influenza viruses (AIVs) cause significant harm to the poultry industry due to mortality as well as high morbidity along with the risk of potential zoonotic transmission to humans.
AIMS
The study aimed to investigate the prevalence of influenza H5, H7, and H9 viruses and their co-infections in layers having respiratory distress such as sneezing, coughing, and tracheal rales.
METHODS
Totally, 960 tracheal swabs (240 swabs in each season) were collected from 120 poultry flocks, including 10 farms per month and 8 samples per flock, located in Karachi where the outbreaks were reported. The samples were confirmed through antigen ELISA and subtyped by RT-PCR.
RESULTS
Antigen ELISA revealed that the prevalence of avian influenza viruses was 26.45%; however, seasonal differences were not significant (P<0.05). RT-PCR subtyping of hemagglutinin (HA) gene revealed the higher prevalence of H9 virus (40.16%) as compared to H7 virus (5.51%) and H5 virus (4.73%). The co-infections comprised H5/H7/H9 (37.0%) and H5/H9 (12.6%).
CONCLUSION
This study shows that AI is endemic in layer farms in Karachi where the H9 subtype is predominant along with co-infections of H5/H7/H9 subtypes.
背景
禽流感病毒（AIVs）因导致死亡率以及高发病率，同时存在向人类进行潜在人畜共患病传播的风险，对家禽业造成重大危害。
目的
本研究旨在调查H5、H7和H9流感病毒在出现如打喷嚏、咳嗽和气管啰音等呼吸窘迫症状的蛋鸡中的流行情况及其合并感染情况。
方法
从卡拉奇报告有疫情的120个家禽鸡群中总共采集了960份气管拭子（每个季节240份拭子），其中每月有10个养殖场，每个鸡群采集8份样本。样本通过抗原ELISA进行确认，并通过RT-PCR进行亚型分型。
结果
抗原ELISA显示禽流感病毒的流行率为26.45%；然而，季节差异不显著（P<0.05）。血凝素（HA）基因的RT-PCR亚型分型显示，H9病毒的流行率（40.16%）高于H7病毒（5.51%）和H5病毒（4.73%）。合并感染包括H5/H7/H9（37.0%）和H5/H9（12.6%）。
结论
本研究表明，禽流感在卡拉奇的蛋鸡养殖场呈地方流行，其中H9亚型占主导地位，同时存在H5/H7/H9亚型的合并感染。
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