McGuire D M, Gross M D, Elde R P, van Pilsum J F
J Histochem Cytochem. 1986 Apr;34(4):429-35. doi: 10.1177/34.4.3512696.
Creatine is a major component of energy metabolism and enzymes involved in its synthesis have therefore been of considerable interest. L-arginine-glycine amidinotransferase, commonly called transamidinase, catalyzes the first reaction in the biosynthesis of creatine. This first reaction is believed to occur in the kidney because of the high concentration of transamidinase in that tissue. Transamidinase activity is also found in many other tissues of the rat, but its role in these tissues is not known. Immunochemical studies with antisera and monoclonal antibodies were used to confirm and refine our understanding of the presence of transamidinase in rat tissues. Immunofluorescence histochemistry was performed to localize transamidinase immunoreactivity within specific tissues including cells in the proximal tubules of the kidney, hepatocytes of the liver, and alpha cells of the pancreatic islet. Immunochemical studies with monoclonal antibodies confirm localization of transamidinase immunoreactivity in the proximal tubules of the kidney. The localization of such immunoreactivity in specialized cells yields insight into possible physiological role(s) of transamidinase in the rat.
肌酸是能量代谢的主要成分，因此参与其合成的酶备受关注。L-精氨酸-甘氨酸脒基转移酶，通常称为转脒基酶，催化肌酸生物合成的第一步反应。由于该组织中转脒基酶的高浓度，人们认为这第一步反应发生在肾脏中。在大鼠的许多其他组织中也发现了转脒基酶活性，但其在这些组织中的作用尚不清楚。使用抗血清和单克隆抗体进行免疫化学研究，以证实和完善我们对大鼠组织中转脒基酶存在情况的理解。进行免疫荧光组织化学以在特定组织内定位转脒基酶免疫反应性，这些组织包括肾近端小管中的细胞、肝脏中的肝细胞和胰岛的α细胞。用单克隆抗体进行的免疫化学研究证实了转脒基酶免疫反应性在肾近端小管中的定位。这种免疫反应性在特化细胞中的定位有助于深入了解转脒基酶在大鼠体内可能的生理作用。
