Gay D, Coeshott C, Golde W, Kappler J, Marrack P
J Immunol. 1986 Mar 15;136(6):2026-32.
Antibody inhibition studies were done to determine which molecules on the surface of the T cell hybridomas other than their receptors for antigen plus IAd were involved in interaction with antigen-presenting B cells, with artificial IAd membranes on glass beads, or with anti-receptor antibodies coupled to Sepharose beads. We found that T cell LFA-1 was only involved when B cells were used to present antigen plus IAd, whereas T cell L3T4 was involved in the response of T cells to antigen plus IAd either on cells or in artificial membranes, but not if anti-receptor antibodies were used to stimulate the T cells. From these results we concluded that LFA-1 may be involved in the recognition of a ligand on cells that was not present in artificial membranes, but that L3T4 might interact with a nonpolymorphic portion of class II molecules present in both intact antigen-presenting cells and the antigen-presenting artificial membranes.
进行了抗体抑制研究，以确定T细胞杂交瘤表面除其抗原加IAd受体外的哪些分子参与与抗原呈递B细胞、玻璃珠上的人工IAd膜或偶联到琼脂糖珠上的抗受体抗体的相互作用。我们发现，仅当使用B细胞呈递抗原加IAd时，T细胞LFA-1才会参与，而T细胞L3T4参与T细胞对细胞上或人工膜上的抗原加IAd的反应，但如果使用抗受体抗体刺激T细胞则不参与。从这些结果我们得出结论，LFA-1可能参与识别细胞上不存在于人工膜中的配体，但L3T4可能与完整抗原呈递细胞和抗原呈递人工膜中都存在的II类分子的非多态部分相互作用。
