Moazezi Ghavihelm Ali, Jamshidi Shahram, Ashrafi Tamai Iraj, Zangisheh Mahsa
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Microbiology, College of Veterinary Medicine, University of Tehran, Tehran, Iran.
JFMS Open Rep. 2022 Jan 30;8(1):20551169211070991. doi: 10.1177/20551169211070991. eCollection 2022 Jan-Jun.
This study was aimed at detecting feline autosomal dominant polycystic kidney disease in a population of Persian and Persian-related breeds by a molecular method in Iran.
Buccal swab samples were collected from 47 cats and examined with a touchdown PCR method. Additionally, partial sequencing was performed in two cats with bilateral renal cysts.
Twenty-two cats (46.8%) were diagnosed as heterozygous for this mutation. Sequence analysis of two cats showed C to A point mutation in the gene, as in previous studies.
Prevalence of this disease is high in Iran, highlighting the need for molecular screening tests before including cats in breeding programmes.
本研究旨在通过分子方法在伊朗的波斯猫及与波斯猫相关品种群体中检测猫常染色体显性多囊肾病。
从47只猫采集颊拭子样本，采用降落式聚合酶链反应方法进行检测。此外，对两只患有双侧肾囊肿的猫进行了部分测序。
22只猫（46.8%）被诊断为该突变的杂合子。两只猫的序列分析显示，与之前的研究一样，该基因存在从C到A的点突变。
该疾病在伊朗的患病率很高，这凸显了在将猫纳入繁殖计划之前进行分子筛查检测的必要性。
Zotero 插件